Roy Rani, Boskey Adele L, Bonassar Lawrence J
Sibley School of Mechanical and Aerospace Engineering, Cornell University, 218 Upson Hall, Ithaca, New York 14853, USA.
J Orthop Res. 2008 Nov;26(11):1434-9. doi: 10.1002/jor.20662.
Collagen glycated with ribose (250 mM) in solution (pre-glycation) and as a gel (post-glycation) was seeded with chondrocytes and the effects of glycation on chondrocyte matrix assembly in culture were determined. Pre-glycation enhanced GAG accumulation significantly over controls at both 2 and 4 weeks (p < 0.05), although at both time points there were no statistical differences in cell number between pre-glycated and control gels. The increased proteoglycan accumulation was shown to be in part due to significantly increased GAG retention by the pre-glycated constructs (p < 0.05). Total collagen content in these pre-glycated gels was also significantly higher than unglycated gels at 4 weeks (p < 0.05). With post-glycation of collagen gels, chondrocyte number and GAG accumulation were all significantly lower than controls (p < 0.05). Post-glycation also inhibited GAG retention by the constructs (p < 0.05). Given these results, pre-glycation may be an improved processing method for collagen gels for tissue engineering techniques.
将溶液中（糖基化前）和凝胶状态下（糖基化后）用核糖（250 mM）糖基化的胶原蛋白接种软骨细胞，测定糖基化对培养中软骨细胞基质组装的影响。糖基化前，在第2周和第4周时，糖胺聚糖（GAG）积累均显著高于对照组（p < 0.05），尽管在这两个时间点，糖基化前和对照凝胶之间的细胞数量没有统计学差异。结果表明，蛋白聚糖积累增加部分是由于糖基化前构建体中GAG保留显著增加（p < 0.05）。在第4周时，这些糖基化前凝胶中的总胶原蛋白含量也显著高于未糖基化凝胶（p < 0.05）。胶原蛋白凝胶糖基化后，软骨细胞数量和GAG积累均显著低于对照组（p < 0.05）。糖基化后也抑制了构建体对GAG的保留（p < 0.05）。鉴于这些结果，糖基化前可能是一种改进的用于组织工程技术的胶原蛋白凝胶加工方法。
