Perez-Iratxeta Carolina, Andrade-Navarro Miguel A
Ontario Genomics Innovation Centre, Ottawa Health Research Institute, 501 Smyth, Ottawa, ON, K1H 8L6, Canada.
BMC Struct Biol. 2008 May 13;8:25. doi: 10.1186/1472-6807-8-25.
Circular dichroism spectroscopy is a widely used technique to analyze the secondary structure of proteins in solution. Predictive methods use the circular dichroism spectra from proteins of known tertiary structure to assess the secondary structure contents of a protein with unknown structure given its circular dichroism spectrum.
We developed K2D2, a method with an associated web server to estimate protein secondary structure from circular dichroism spectra. The method uses a self-organized map of spectra from proteins with known structure to deduce a map of protein secondary structure that is used to do the predictions.
The K2D2 server is publicly accessible at http://www.ogic.ca/projects/k2d2/. It accepts as input a circular dichroism spectrum and outputs the estimated secondary structure content (alpha-helix and beta-strand) of the corresponding protein, as well as an estimated measure of error.
圆二色光谱法是一种广泛用于分析溶液中蛋白质二级结构的技术。预测方法利用已知三级结构蛋白质的圆二色光谱，根据未知结构蛋白质的圆二色光谱来评估其二级结构含量。
我们开发了K2D2，一种带有相关网络服务器的方法，用于从圆二色光谱估计蛋白质二级结构。该方法使用已知结构蛋白质光谱的自组织映射来推导用于预测的蛋白质二级结构映射。
K2D2服务器可通过http://www.ogic.ca/projects/k2d2/公开访问。它接受圆二色光谱作为输入，并输出相应蛋白质的估计二级结构含量（α-螺旋和β-链）以及估计的误差度量。
