Nigham Anshul, Tucker-Kellogg Lisa, Mihalek Ivana, Verma Chandra, Hsu David
Department of Computer Science, National University of Singapore, Singapore 117590, Singapore-MIT Alliance, Singapore.
Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W246-51. doi: 10.1093/nar/gkn259. Epub 2008 May 13.
The pFlexAna (protein flexibility analyzer) web server detects and displays conformational changes in remotely related proteins, without relying on sequence homology. To do so, it first applies a reliable statistical test to align core protein fragments that are structurally similar and then clusters these aligned fragment pairs into 'super-alignments', according to the similarity of geometric transformations that align them. The result is that the dominant conformational changes occur between the clusters, while the smaller conformational changes occur within a cluster. pFlexAna is available at http://bigbird.comp.nus.edu.sg/pfa2/.
pFlexAna（蛋白质灵活性分析器）网络服务器可检测并显示远缘相关蛋白质中的构象变化，而无需依赖序列同源性。为此，它首先应用可靠的统计测试来比对结构相似的核心蛋白质片段，然后根据比对这些片段的几何变换的相似性，将这些比对的片段对聚类为“超级比对”。结果是，主要的构象变化发生在聚类之间，而较小的构象变化发生在一个聚类内部。可通过http://bigbird.comp.nus.edu.sg/pfa2/访问pFlexAna。
