Munisamy S, Vaidyanathan T K, Vaidyanathan J
New Jersey Dental School, University of Medicine and Dentistry of New Jersey, 110 Bergen Street, Newark, NJ 07103, USA.
J Oral Implantol. 2008;34(2):67-75. doi: 10.1563/1548-1336(2008)34[67:ABPSFI]2.0.CO;2.
Many surface modification strategies are currently of interest in improving integration of implants to bone. An in vitro precoating of a bone-like mineralized layer of immobilized collagen on the implant surface is a potentially valuable approach to improve host acceptance of the implant. The goal of this investigation was to develop a method to precoat in vitro a bone-like mineralized collagen layer on a pure titanium dental implant surface. The study was conducted on acid-etched and nonetched surfaces of screw implants. Initially, a procedure was standardized to self-assemble collagen from a collagen solution. In subsequent experiments, the implant was also placed inside the solution, and after 3 days, collagen was found to be coated on the implant surface. Mineralization of the collagen gel as well as collagen coating on the implant was carried out by calcium phosphate precipitation from a mineralizing solution of calcium chloride containing polyvinyl phosphonic acid and polyaspartic acid, which served as polyanionic additives to help disperse the precipitation and template mineral nucleation. The implant was kept in the mineralizing solution and maintained for 2 weeks in an incubator at 37 degrees C with a phosphate vapor phase generated from a vial containing dihydrogen ammonium phosphate in the incubator. Scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy analysis confirmed the coated layer to be a biomimetic bone-like mineralized type 1 collagen. Initial studies using osteoblast-like cells indicated cellular attachment on the modified surface. The method appears to be a promising way to generate in vitro a bone-like layer on the implant surface.
目前，许多表面改性策略都有助于改善植入物与骨的整合。在植入物表面体外预涂覆一层固定化胶原蛋白的类骨矿化层，是提高植入物宿主接受度的一种潜在有价值的方法。本研究的目的是开发一种在纯钛牙科植入物表面体外预涂覆类骨矿化胶原蛋白层的方法。该研究在螺丝形植入物的酸蚀和未蚀刻表面上进行。最初，将一种从胶原蛋白溶液中自组装胶原蛋白的程序标准化。在后续实验中，还将植入物置于溶液中，3天后，发现胶原蛋白已涂覆在植入物表面。通过氯化钙矿化溶液中的磷酸钙沉淀来实现胶原蛋白凝胶以及植入物上胶原蛋白涂层的矿化，该溶液含有聚乙烯膦酸和聚天冬氨酸，它们作为聚阴离子添加剂，有助于分散沉淀并模板化矿物成核。将植入物置于矿化溶液中，并在37℃的培养箱中保持2周，培养箱中有一个装有磷酸二氢铵的小瓶产生的磷酸盐气相。扫描电子显微镜、X射线衍射和傅里叶变换红外光谱分析证实涂覆层为仿生类骨矿化I型胶原蛋白。使用类成骨细胞的初步研究表明细胞附着在改性表面上。该方法似乎是在体外在植入物表面生成类骨层的一种有前景的方法。
