Zeleznik-Le N J, Azizkhan J C, Ting J P
Lineberger Cancer Research Center, University of North Carolina, Chapel Hill 27599.
Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1873-7. doi: 10.1073/pnas.88.5.1873.
Efficient major histocompatibility complex class II gene expression requires conserved protein-binding promoter elements, including X and Y elements. We affinity purified an HLA-DRA Y-element (CCAAT)-binding protein (YEBP) and used it to reconstitute Y-depleted HLA-DRA in vitro transcription. This directly demonstrates a positive functional role for YEBP in HLA-DRA transcription. The ability of YEBP to regulate divergent CCAAT elements was also assessed; YEBP was found to partially activate the thymidine kinase promoter. This functional analysis of YEBP shows that this protein plays an important role in the regulation of multiple genes.
高效的主要组织相容性复合体II类基因表达需要保守的蛋白质结合启动子元件，包括X和Y元件。我们通过亲和纯化得到了一种与HLA-DRA Y元件（CCAAT）结合的蛋白（YEBP），并用它在体外转录中重建了Y元件缺失的HLA-DRA。这直接证明了YEBP在HLA-DRA转录中具有正向功能作用。我们还评估了YEBP调节不同CCAAT元件的能力；发现YEBP能部分激活胸苷激酶启动子。对YEBP的这种功能分析表明，该蛋白在多个基因的调控中发挥着重要作用。
