Looney W B, Schaffner J G, Trefil J S, Kovacs C J, Hopkins H A
Br J Cancer. 1976 Sep;34(3):254-61. doi: 10.1038/bjc.1976.160.
Neither radiation alone (375 to 1500 rad) nor5-fluorouracil (FU) alone (50-250 mg/kg) is sufficient to prevent an increase in the volume of the solid tumour model hepatoma 3924A. However, as little as 750 rad with 100 mg/kg FU can reduce the tumour below the volume at the time of treatment for as long as 14 days. A series of combined FU and radiation doses given every 11 days should then result in successively smaller tumour volumes until the tumour is eradicated. Changes in tumour volume were analysed by two different methods: (1) tumours in each treatment mode were grouped together and the average response to treatment determined, and (2) tumour volume changes in individual tumours were analyzed utilizing the chi2 technique, which fits the logarithmic tumour volume change with time to polynomials. This two-directional method of analysis has the advantage of permitting both an overview of the main effects of treatment via the averages, and at the same time a detailed examination of the mechanism by which these effects occur through the analysis of individual response. The results suggest that, in addition to concentrating on the cellular response immediately after therapy, greater emphasis should be placed on the kinetic changes of the tumour 1-3 weeks after single or multiple modality therapy. These findings demonstrate how the sequencing of single and/or combined treatment modalities may be investigated in order to detemine how best to obtain maximum effects of treatment on different types of tumours following recovery of the host from the previous treatment series.
单独使用辐射（375至1500拉德）或单独使用5-氟尿嘧啶（FU，50 - 250毫克/千克）都不足以阻止实体瘤模型肝癌3924A的体积增加。然而，低至750拉德的辐射与100毫克/千克的FU联合使用，可使肿瘤体积在治疗后至少14天内保持低于治疗时的体积。每11天给予一系列联合的FU和辐射剂量，应能使肿瘤体积逐渐减小，直至肿瘤被根除。通过两种不同方法分析肿瘤体积的变化：（1）将每种治疗模式下的肿瘤归为一组，确定对治疗的平均反应；（2）利用卡方技术分析单个肿瘤的体积变化，该技术将肿瘤体积随时间的对数变化拟合为多项式。这种双向分析方法的优点是，既能通过平均值对治疗的主要效果有一个总体了解，同时又能通过对个体反应的分析详细研究这些效果产生的机制。结果表明，除了关注治疗后立即出现的细胞反应外，还应更加重视单模态或多模态治疗后1至3周肿瘤的动力学变化。这些发现展示了如何研究单一和/或联合治疗模式的顺序，以便确定在宿主从上一个治疗系列恢复后，如何以最佳方式获得对不同类型肿瘤的最大治疗效果。
