Zhai Hongbo, Cordoba-Diaz Manuel, Wa Chrystal, Hui Xiaoying, Maibach Howard I
Department of Dermatology, University of California, San Francisco, San Francisco, CA 94143-0989, USA.
J Cosmet Dermatol. 2008 Jun;7(2):96-100. doi: 10.1111/j.1473-2165.2008.00370.x.
BACKGROUND/AIMS: The goal of this study was to determine the ability of two putative antioxidant solutions to suppress the formation of superoxide anion radicals created by optical excitation of a photosensitizer and measured by highly sensitive luminometric detection.
METHODS/RESULTS: Solutions containing 3% antioxidant complex and 1% idebenone were tested. The antioxidant complex is an aqueous combination of botanical extracts rich in ferulic acid, caffeic acid, and other polyphenols. Idebenone is a lower molecular weight analog of coenzyme Q(10) (a potent antioxidant). Each was dissolved in lipid soluble reagents (solutions) and run through a Photochem (photochemiluminometer system) device. Their antioxidant capacity was expressed as nmol equivalents of synthetic vitamin E. Nine aliquots of each compound were measured. The mean antioxidant capacities for the 3% antioxidant complex and 1% idebenone were 525 +/- 23 and 213 +/- 14 nmol, respectively, with a statistically significant difference between the two compounds (P < 0.001).
The 3% antioxidant solution possesses a significant antioxidative capacity, which was 2.5 times greater than the known antioxidant idebenone in a 1% solution as shown by the quenching of superoxide anion radicals measured by photochemiluminescence. This method provides rapid, accurate, and sensitive measurement of the antioxidant properties of lipid-soluble compounds.
背景/目的：本研究的目的是确定两种假定的抗氧化剂溶液抑制由光敏剂光激发产生并通过高灵敏度发光检测测量的超氧阴离子自由基形成的能力。
方法/结果：测试了含有3%抗氧化剂复合物和1%艾地苯醌的溶液。抗氧化剂复合物是富含阿魏酸、咖啡酸和其他多酚的植物提取物的水性组合。艾地苯醌是辅酶Q(10)（一种有效的抗氧化剂）的低分子量类似物。每种都溶解在脂溶性试剂（溶液）中，并通过光化学（光化学发光计系统）装置运行。它们的抗氧化能力以合成维生素E的纳摩尔当量表示。每种化合物测量了9份等分试样。3%抗氧化剂复合物和1%艾地苯醌的平均抗氧化能力分别为525±23和213±14纳摩尔，两种化合物之间存在统计学显著差异（P<0.001）。
3%抗氧化剂溶液具有显著的抗氧化能力，通过光化学发光测量超氧阴离子自由基的猝灭表明，其抗氧化能力是1%溶液中已知抗氧化剂艾地苯醌的2.5倍。该方法提供了对脂溶性化合物抗氧化性能的快速、准确和灵敏的测量。
