采用抑制化学发光检测流动注射分析法测定飞蓬提取物及药物制剂中的酚类化合物及其抗氧化活性。
Determination of phenolic compounds and their antioxidant activity in Erigeron acris L. extracts and pharmaceutical formulation by flow injection analysis with inhibited chemiluminescent detection.
作者信息
Nalewajko-Sieliwoniuk Edyta, Nazaruk Jolanta, Antypiuk Ewelina, Kojło Anatol
机构信息
Institute of Chemistry, University of Białystok, ul. Hurtowa 1, 15-399 Białystok, Poland.
出版信息
J Pharm Biomed Anal. 2008 Nov 4;48(3):579-86. doi: 10.1016/j.jpba.2008.05.026. Epub 2008 May 28.
It was found that the chemiluminescence (CL) produced from the reaction of luminol with iodine in the alkaline medium was strongly inhibited by plant phenolic compounds. Based on this finding, a new flow injection CL method was developed for the determination of caffeic acid and 6'-caffeoylerigeroside. The latter compound was isolated for the first time from Erigeron acris L. herb. The method was simple, rapid and sensitive with a detection limit of 4 x 10(-3) ng mL(-1) (caffeic acid) and 0.18 ng mL(-1) (6'-caffeoylerigeroside), linear range of 0.1-1.5 ng mL(-1) (caffeic acid) and 1-200 ng mL(-1) (6'-caffeoylerigeroside), relative standard deviation of 3.3% for 10 measurements of 0.45 ng mL(-1) caffeic acid and 2.9% for 40 ng mL(-1) 6'-caffeoylerigeroside. This method was successfully applied to determine the content of phenolic compounds/antioxidant activity of E. acris L. extracts and phenolic acids content in pharmaceutical formulation. A possible mechanism of the inhibition of the proposed CL system was discussed.
研究发现，鲁米诺与碘在碱性介质中反应产生的化学发光（CL）受到植物酚类化合物的强烈抑制。基于这一发现，开发了一种新的流动注射化学发光法用于测定咖啡酸和6'-咖啡酰基紫菀苷。后者化合物首次从飞蓬属植物草本中分离得到。该方法简单、快速且灵敏，咖啡酸的检测限为4×10⁻³ ng mL⁻¹，6'-咖啡酰基紫菀苷的检测限为0.18 ng mL⁻¹；咖啡酸的线性范围为0.1 - 1.5 ng mL⁻¹，6'-咖啡酰基紫菀苷的线性范围为1 - 200 ng mL⁻¹；对0.45 ng mL⁻¹咖啡酸进行10次测量的相对标准偏差为3.3%，对40 ng mL⁻¹ 6'-咖啡酰基紫菀苷进行40次测量的相对标准偏差为2.9%。该方法成功应用于测定飞蓬属植物提取物中酚类化合物的含量/抗氧化活性以及药物制剂中酚酸的含量。并讨论了所提出的化学发光体系抑制的可能机制。
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