Tynngård Nahreen, Studer Monika, Lindahl Tomas L, Trinks Marie, Berlin Gösta
Department of Clinical Immunology and Transfusion Medicine, University Hospital, Linköping, Sweden.
Transfusion. 2008 Aug;48(8):1669-75. doi: 10.1111/j.1537-2995.2008.01746.x. Epub 2008 May 14.
This study compares the quality of gamma-irradiated versus nonirradiated platelet (PLT) concentrates (PCs) during storage for 7 days as assessed by various in vitro methods. A new technique, free oscillation rheometry (FOR), which measures clotting time and coagulum elasticity, was also used to evaluate the PLT function.
Single-donor PLTs were collected by apheresis technique (n = 20). The PLTs from each donor were divided into two PCs, one gamma-irradiated with 25 Gy and the other used as a nonirradiated control. Blood gases, metabolic variables, and swirling were analyzed from Day 0. Samples taken on Days 1, 5, and 7 were also analyzed for hypotonic shock response (HSR), P-selectin, and glycoprotein (GP)Ib expression by flow cytometry and coagulation by FOR.
Swirling, HSR, and the percentage of GPIb-expressing cells were well maintained for 7 days of storage. pH was always within accepted range (6.4-7.4). Glucose decreased and lactate increased during the storage period (p < 0.05). P-selectin expression increased during storage (p < 0.05). The FOR clotting time remained constant, whereas the build-up of elasticity was slower after storage (p < 0.05). No difference was found between irradiated and nonirradiated PCs.
The results indicate a well-preserved quality of gamma-irradiated apheresis PLTs during storage for 7 days as assessed by in vitro methods, with no difference compared to nonirradiated PLTs.
本研究通过多种体外方法比较了γ射线辐照与未辐照的血小板浓缩物（PCs）在储存7天期间的质量。还使用了一种测量凝血时间和凝块弹性的新技术——自由振荡流变学（FOR）来评估血小板功能。
通过单采技术收集单供者血小板（n = 20）。将每个供者的血小板分成两份PCs，一份用25 Gy的γ射线辐照，另一份作为未辐照对照。从第0天开始分析血气、代谢变量和漩涡情况。还对第1天、第5天和第7天采集的样本进行分析，通过流式细胞术检测低渗休克反应（HSR）、P-选择素和糖蛋白（GP）Ib表达，并通过FOR检测凝血情况。
在储存7天期间，漩涡情况、HSR和表达GPIb的细胞百分比保持良好。pH始终在可接受范围内（6.4 - 7.4）。储存期间葡萄糖降低，乳酸增加（p < 0.05）。储存期间P-选择素表达增加（p < 0.05）。FOR凝血时间保持恒定，而储存后弹性的增强较慢（p < 0.05）。辐照和未辐照的PCs之间未发现差异。
结果表明，通过体外方法评估，γ射线辐照的单采血小板在储存7天期间质量保存良好，与未辐照的血小板相比无差异。
