Departamento de Apoio, Produgao e Saiide Animal, da Faculdade de Odontologia, Universidade Estadual Paulista, Aragatuba.
Avian Pathol. 1998;27(5):450-4. doi: 10.1080/03079459808419368.
A double antibody sandwich ELISA (DAS-ELISA) was developed and employed for simultaneous direct detection of infectious bursal disease virus (IBDV) from bursal samples and to measure the humoral response, using the same basic immunoreagents. The purified and non-purified antigen, capture antibody and chicken hyperimmune sera were prepared, and standardized for this purpose. The DAS-ELISA was applied to both 80 bursal suspensions and 224 corresponding serum samples from vaccinated and non-vaccinated commercial flocks. Bursae samples were collected at 2 weeks of age, and submitted to histological examination, virus isolation in specific pathogen-free chickens embryos, and the DAS-ELISA technique. Serum titres obtained in indirect ELISA and serum neutralization test were compared with those in DAS-ELISA. The agreement was 80% between DAS-ELISA, and the conventional techniques, with high sensitivity (87%) and specificity (90%).
建立了一种双抗体夹心 ELISA（DAS-ELISA），并使用相同的基本免疫试剂，用于从法氏囊样本中同时直接检测传染性法氏囊病病毒（IBDV）并测量体液免疫反应。为此目的，制备了纯化和未纯化的抗原、捕获抗体和鸡高免血清，并对其进行了标准化。该 DAS-ELISA 应用于 80 个法氏囊悬浮液和 224 个来自接种和未接种商业鸡群的相应血清样本。在 2 周龄时采集法氏囊样本，并进行组织学检查、在无特定病原体鸡胚中分离病毒以及 DAS-ELISA 技术。将间接 ELISA 和血清中和试验中获得的血清效价与 DAS-ELISA 进行比较。DAS-ELISA 与传统技术的一致性为 80%，具有较高的敏感性（87%）和特异性（90%）。
