Moro de Sousa R L, Montassier H J, Pinto A A
Graduate Program in Microbiology, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
Clin Diagn Lab Immunol. 2000 Nov;7(6):940-4. doi: 10.1128/CDLI.7.6.940-944.2000.
A liquid phase blocking ELISA (LPB-ELISA) was adapted for the detection and quantification of antibodies to Newcastle disease virus. Sera from vaccinated and unvaccinated commercial flocks of ostriches (Struthio camelus) and rheas (Rhea americana) were tested. The purified and nonpurified virus used as the antigen and the capture and detector antibodies were prepared and standardized for this purpose. The hemagglutination-inhibition (HI) test was regarded as the reference method. The cutoff point for the LPB-ELISA was determined by a two-graph receiver operating characteristic analysis. The LPB-ELISA titers regressed significantly (P < 0.0001) on the HI titers with a high correlation coefficient (r = 0.875). The two tests showed good agreement (kappa = 0.82; P < 0.0001), relative sensitivity (90.91%) and specificity (91.18%), and accuracy (91.02%), suggesting that they are interchangeable.
一种液相阻断酶联免疫吸附测定法（LPB - ELISA）被用于检测和定量新城疫病毒抗体。对接种和未接种疫苗的商业鸵鸟（鸵鸟属）和美洲鸵鸟（美洲鸵鸟属）群体的血清进行了检测。为此制备并标准化了用作抗原的纯化和未纯化病毒以及捕获抗体和检测抗体。血凝抑制（HI）试验被视为参考方法。通过双图受试者工作特征分析确定了LPB - ELISA的临界值。LPB - ELISA滴度与HI滴度显著相关（P < 0.0001），相关系数较高（r = 0.875）。这两种检测方法显示出良好的一致性（kappa = 0.82；P < 0.0001）、相对灵敏度（90.91%）、特异性（91.18%）和准确性（91.02%），表明它们具有互换性。
