Hagiwara Yoshihiro, Chimoto Eiichi, Ando Akira, Sasano Yasuyuki, Hatori Kouki
Department of Orthopaedic Surgery, Tohoku University Graduate School of Medicine, 1-1 Seiryomachi, Aobaku, Sendai, Japan.
Ups J Med Sci. 2007;112(3):356-65. doi: 10.3109/2000-1967-208.
Contracture is a very common complication in daily examination and a fibrotic change of a capsule is suggested to be a one of the main causes. Type I collagen is a major component of a synovial capsule and also has been implicated in tissue elasticity of other organs. We immobilized the knee joints of 66 rats in 150 degrees of flexion using a plastic plate and metal screws. Sham operated knee joints had holes drilled and screws inserted but none of them were plated. The expression patterns of type I collagen were characterized using in situ hybridization and immunohistochemistry. The in situ hybridization demonstrated that the mRNA of type I collagen decreased rapidly after immobilization. However, the immunoreactivity of the capsule was not changed in the immobilized and the control groups at any time points. Other processes might be considered to evaluate the contracture capsule.
挛缩是日常检查中非常常见的并发症，滑膜囊的纤维化改变被认为是主要原因之一。I型胶原蛋白是滑膜囊的主要成分，也与其他器官的组织弹性有关。我们使用塑料板和金属螺钉将66只大鼠的膝关节固定在150度屈曲位。假手术的膝关节钻孔并插入螺钉，但均未进行固定。使用原位杂交和免疫组织化学对I型胶原蛋白的表达模式进行了表征。原位杂交表明，固定后I型胶原蛋白的mRNA迅速减少。然而，在任何时间点，固定组和对照组的滑膜囊免疫反应性均未改变。可能需要考虑其他过程来评估挛缩的滑膜囊。
