Department of Orthopaedic Surgery, Tohoku University School of Medicine, Sendai; Takeda General Hospital, Aizuwakamatsu, Japan.
Connect Tissue Res. 2010;51(1):22-30. doi: 10.3109/03008200902859406.
The purpose of our study was to clarify the expression patterns of collagen types I and II on articular cartilage after immobilization in a rat knee contracture model in 3 specific areas (noncontact area, transitional area, contact area). The unilateral knee joints of adult male rats were rigidly immobilized at 150 degrees of flexion using screws and a rigid plastic plate. Sham-operated animals had holes drilled in the femur and the tibia and screws inserted but were not plated. The expression patterns of collagen types I and II in each area were evaluated by in situ hybridization (ISH), immunohistochemistry (IHC), and quantitative real-time polymerase chain reaction (qPCR). The expression of collagen type II in the noncontact area was decreased by ISH but appeared unchanged when examined by IHC. In the transitional and contact areas, the expression of collagen type II was initially shown to have decreased and then increased at the hypertrophic chondrocytes by ISH but appeared decreased by IHC. Quantitative PCR revealed the decreased expression of type II collagen in the contact area. Immunostaining of collagen type I was increased at the noncontact area and transitional areas. Alterations of collagen types I and II expression may also affect the degeneration of articular cartilage after immobilization and the changes were different in the three areas.
我们的研究目的是阐明在大鼠膝关节挛缩模型中，关节软骨在 3 个特定区域（非接触区、过渡区、接触区）固定后的 I 型和 II 型胶原的表达模式。使用螺钉和刚性塑料板将成年雄性大鼠的单侧膝关节固定在 150 度的弯曲位置，使其处于刚性固定状态。假手术组动物的股骨和胫骨钻孔，并插入螺钉，但不使用固定板。通过原位杂交（ISH）、免疫组织化学（IHC）和实时定量聚合酶链反应（qPCR）评估每个区域中 I 型和 II 型胶原的表达模式。ISH 显示非接触区的 II 型胶原表达减少，但 IHC 检查时未见明显变化。在过渡区和接触区，ISH 显示 II 型胶原的表达最初减少，然后在肥大软骨细胞中增加，但 IHC 显示减少。qPCR 显示接触区 II 型胶原表达减少。I 型胶原的免疫染色在非接触区和过渡区增加。I 型和 II 型胶原表达的改变也可能影响固定后关节软骨的退化，并且在三个区域中的变化是不同的。
