Kubonishi I, Daibata M, Yano S, Isobe M, Kurosawa N, Nagumo H, Ogita Z, Ohyashiki J H, Toyama K, Miyoshi I
Department of Medicine, Kochi Medical School, Kochi, Japan.
Am J Hematol. 1991 Jul;37(3):179-85. doi: 10.1002/ajh.2830370309.
A new Epstein-Barr virus nuclear antigen (EBNA)-positive B-cell line, designated BALL-2, was spontaneously established from the peripheral blood of a 14-year-old boy with an EBNA-negative B-cell acute lymphoblastic leukemia (B-ALL), L2 in the French-American-British classification. The BALL-2 cell line grew in suspension with or without forming clumps of cells. The cultured cells exhibited lymphoid morphology with indented or lobulated nuclei, prominent nucleoli, and relatively abundant cytoplasm. Immunologic and cytogenetic studies showed that the BALL-2 cell line expressed the B-cell phenotype, CpIg+, SmIg+, CD19+, CD20+, CD38-, Ia+, and had chromosome translocation, t(8;14) (q24;q32). The same phenotypic and chromosome markers were present in original leukemia cells. These results indicated that the cell line was derived from the patient's leukemia cells. Unexpectedly, however, BALL-2 cells were positive for EBNA and EB virus DNA. Gene analysis of the BALL-2 cell line showed biallelic rearrangements in the JH locus. One of the JH rearrangement comigrated with a rearranged c-myc gene, indicating the translocation had occurred between JH and c-myc loci. The t(8;14) abnormality is a known chromosome marker of Burkitt lymphoma and L3 type ALL. Our studies revealed that this translocation and myc gene rearrangement can also be found in L2 type B-ALL.
一种新的爱泼斯坦-巴尔病毒核抗原（EBNA）阳性B细胞系，命名为BALL-2，是从一名14岁患EBNA阴性B细胞急性淋巴细胞白血病（B-ALL）（法国-美国-英国分类中的L2型）男孩的外周血中自发建立的。BALL-2细胞系在悬浮状态下生长，有无细胞团块形成。培养的细胞呈现淋巴细胞形态，核有凹陷或分叶，核仁突出，细胞质相对丰富。免疫和细胞遗传学研究表明，BALL-2细胞系表达B细胞表型，CpIg+、SmIg+、CD19+、CD20+、CD38-、Ia+，并具有染色体易位t(8;14)(q24;q32)。原始白血病细胞中也存在相同的表型和染色体标记。这些结果表明该细胞系源自患者的白血病细胞。然而，出乎意料的是，BALL-2细胞EBNA和EB病毒DNA呈阳性。对BALL-2细胞系的基因分析显示JH基因座存在双等位基因重排。其中一种JH重排与重排的c-myc基因共迁移，表明JH和c-myc基因座之间发生了易位。t(8;14)异常是伯基特淋巴瘤和L3型ALL已知的染色体标记。我们的研究表明，这种易位和myc基因重排也可在L2型B-ALL中发现。
