Lee Eujin, Kim Sun-Gun, Park Na-Young, Park Hyo-Hyun, Jeong Kyu-Tae, Choi Jongkeun, Lee In-Hae, Lee Hwadong, Kim Keuk-Jun, Lee Eunkyung
Research and Development Division, National Development Institute of Korean Medicine, Gyeongsan, 712-260, Republic of Korea.
Department of Cosmetic Science, Chungwoon University, Chungnam, 350-701, Republic of Korea.
BMC Complement Altern Med. 2016 Jun 6;16:169. doi: 10.1186/s12906-016-1155-4.
The ethanol extract of KOTMIN13, composed of Inula japonica Flowers, Trichosanthes kirilowii Semen, Peucedanum praeruptorum Radix, and Allium macrostemon Bulbs, was investigated for its anti-asthmatic and anti-allergic activities.
The anti-asthmatic effects of KOTMIN13 were evaluated on ovalbumin (OVA)-induced murine asthma model. Anti-allergic properties of KOTMIN13 in bone-marrow derived mast cells (BMMC) and passive cutaneous anaphylaxis (PCA) in vivo were also examined.
In asthma model, KOTMIN13 effectively suppressed airway hyperresponsiveness induced by aerosolized methacholine when compared to the levels of OVA-induced mice. KOTMIN13 treatment reduced the total leukocytes, eosinophil percentage, and Th2 cytokines in the bronchoalveolar lavage fluids in OVA-induced mice. The increased levels of eotaxin and Th2 cytokines in the lung as well as serum IgE were decreased by KOTMIN13. The histological analysis shows that the increased inflammatory cell infiltration and mucus secretion were also reduced. In addition, the degranulation and leukotriene C4 production were inhibited in BMMC with IC50 values of 3.9 μg/ml and 1.7 μg/ml, respectively. Furthermore, KOTMIN13 treatment attenuated mast-mediated PCA reaction.
These results demonstrate that KOTMIN13 has anti-asthmatic and anti-allergic effects in vivo and in vitro models.
由旋覆花、瓜蒌子、前胡和薤白组成的KOTMIN13乙醇提取物被研究其抗哮喘和抗过敏活性。
在卵清蛋白（OVA）诱导的小鼠哮喘模型上评估KOTMIN13的抗哮喘作用。还检测了KOTMIN13在骨髓来源的肥大细胞（BMMC）中的抗过敏特性以及体内被动皮肤过敏反应（PCA）。
在哮喘模型中，与OVA诱导的小鼠相比，KOTMIN13能有效抑制雾化乙酰甲胆碱诱导的气道高反应性。KOTMIN13治疗降低了OVA诱导小鼠支气管肺泡灌洗液中的总白细胞、嗜酸性粒细胞百分比和Th2细胞因子。KOTMIN13降低了肺中嗜酸性粒细胞趋化因子和Th2细胞因子以及血清IgE的升高水平。组织学分析表明，增加的炎症细胞浸润和黏液分泌也减少了。此外，KOTMIN13在BMMC中抑制脱颗粒和白三烯C4的产生，IC50值分别为3.9μg/ml和1.7μg/ml。此外，KOTMIN13治疗减轻了肥大细胞介导的PCA反应。
这些结果表明KOTMIN13在体内和体外模型中均具有抗哮喘和抗过敏作用。
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