紫花堇菜（VM）醇提物对变应性哮喘小鼠气道炎症的抗炎和平喘作用。
Anti-inflammatory and anti-asthmatic effects of Viola mandshurica W. Becker (VM) ethanolic (EtOH) extract on airway inflammation in a mouse model of allergic asthma.
机构信息
Immune Modulator Research Center, Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yusung, Daejeon 305-600, Republic of Korea.
出版信息
J Ethnopharmacol. 2010 Jan 8;127(1):159-64. doi: 10.1016/j.jep.2009.09.033. Epub 2009 Sep 25.
AIM OF THE STUDY
We investigated the efficacy of Viola mandshurica W. Becker (VM) ethanolic (EtOH) extract in the treatment of bronchial asthma in an ovalbumin (OVA)-induced asthmatic BALB/c mouse model.
MATERIALS AND METHODS
Female BALB/c mice were sensitized with intraperitoneal (i.p.) ovalbumin (OVA) on days 0 and 14, and were next given intranasal OVA on days 28-30. Randomized treatment groups of sensitized mice received VM EtOH extract, dexamethasone, or placebo, orally, from days 28 to 30.
RESULTS
VM EtOH extract significantly inhibited increases in total immunoglobulin E (IgE) and cytokines IL-4 and IL-13 levels in serum and bronchoalveolar lavage fluid (BALF), and also effectively suppressed airway hyperresponsiveness (AHR), eosinophilia, and mucus hypersecretion, in mice with OVA-induced asthma.
CONCLUSIONS
The results suggest that VM EtOH extract and allied extracts could be useful herbal medicines for asthma treatment, and that VM may also be a valuable lead material for anti-asthma drug development.
研究目的
本研究旨在探讨堇菜（Viola mandshurica W. Becker）醇提物（VM）治疗卵清蛋白（OVA）诱导的哮喘 BALB/c 小鼠模型中支气管哮喘的疗效。
材料与方法
雌性 BALB/c 小鼠于第 0 天和第 14 天通过腹腔内（i.p.）注射卵清蛋白（OVA）进行致敏，然后在第 28-30 天通过鼻腔内给予 OVA。致敏小鼠的随机治疗组于第 28 天至第 30 天口服 VM 醇提物、地塞米松或安慰剂。
结果
VM 醇提物可显著抑制 OVA 诱导的哮喘小鼠血清和支气管肺泡灌洗液（BALF）中总免疫球蛋白 E（IgE）和细胞因子 IL-4 和 IL-13 水平的升高，同时还能有效抑制气道高反应性（AHR）、嗜酸性粒细胞增多和黏液高分泌。
结论
结果表明，VM 醇提物和相关提取物可能是治疗哮喘的有用草药，而 VM 也可能是抗哮喘药物开发的有价值的先导材料。
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