Immune Modulator Research Center, Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yusung, Daejeon 305-600, Republic of Korea.
J Ethnopharmacol. 2010 Jan 8;127(1):159-64. doi: 10.1016/j.jep.2009.09.033. Epub 2009 Sep 25.
We investigated the efficacy of Viola mandshurica W. Becker (VM) ethanolic (EtOH) extract in the treatment of bronchial asthma in an ovalbumin (OVA)-induced asthmatic BALB/c mouse model.
Female BALB/c mice were sensitized with intraperitoneal (i.p.) ovalbumin (OVA) on days 0 and 14, and were next given intranasal OVA on days 28-30. Randomized treatment groups of sensitized mice received VM EtOH extract, dexamethasone, or placebo, orally, from days 28 to 30.
VM EtOH extract significantly inhibited increases in total immunoglobulin E (IgE) and cytokines IL-4 and IL-13 levels in serum and bronchoalveolar lavage fluid (BALF), and also effectively suppressed airway hyperresponsiveness (AHR), eosinophilia, and mucus hypersecretion, in mice with OVA-induced asthma.
The results suggest that VM EtOH extract and allied extracts could be useful herbal medicines for asthma treatment, and that VM may also be a valuable lead material for anti-asthma drug development.
本研究旨在探讨堇菜（Viola mandshurica W. Becker）醇提物（VM）治疗卵清蛋白（OVA）诱导的哮喘 BALB/c 小鼠模型中支气管哮喘的疗效。
雌性 BALB/c 小鼠于第 0 天和第 14 天通过腹腔内（i.p.）注射卵清蛋白（OVA）进行致敏，然后在第 28-30 天通过鼻腔内给予 OVA。致敏小鼠的随机治疗组于第 28 天至第 30 天口服 VM 醇提物、地塞米松或安慰剂。
VM 醇提物可显著抑制 OVA 诱导的哮喘小鼠血清和支气管肺泡灌洗液（BALF）中总免疫球蛋白 E（IgE）和细胞因子 IL-4 和 IL-13 水平的升高，同时还能有效抑制气道高反应性（AHR）、嗜酸性粒细胞增多和黏液高分泌。
结果表明，VM 醇提物和相关提取物可能是治疗哮喘的有用草药，而 VM 也可能是抗哮喘药物开发的有价值的先导材料。
