卡氏棘阿米巴：肌动蛋白细胞骨架的鉴定与分布
Acanthamoeba castellanii: identification and distribution of actin cytoskeleton.
作者信息
González-Robles Arturo, Castañón Guadalupe, Hernández-Ramírez Verónica Ivonne, Salazar-Villatoro Lizbeth, González-Lázaro Mónica, Omaña-Molina Maritza, Talamás-Rohana Patricia, Martínez-Palomo Adolfo
机构信息
Department of Experimental Pathology, Center for Research and Advanced Studies, Mexico City, Mexico.
出版信息
Exp Parasitol. 2008 Jul;119(3):411-7. doi: 10.1016/j.exppara.2008.04.004. Epub 2008 Apr 12.
The presence of the cytoskeleton of Acanthamoeba castellanii was observed by means of cryo-electronmicroscopy and immunofluorescence techniques. This structure is formed largely by fibers and networks of actin located mainly in cytoplasmic locomotion structures as lamellipodia and as well as in various endocytic structures. In addition, the comparison between total actin content in whole extracts among different amoebae was made. The molecular weight of actin in A. castellanii was 44 kDa, and 45 kDa for Naegleria fowleri and Entamoeba histolytica.
通过冷冻电子显微镜和免疫荧光技术观察了卡氏棘阿米巴的细胞骨架。这种结构主要由肌动蛋白纤维和网络形成，主要位于细胞质运动结构如片状伪足以及各种内吞结构中。此外，还对不同变形虫全提取物中的总肌动蛋白含量进行了比较。卡氏棘阿米巴肌动蛋白的分子量为44 kDa，福氏耐格里阿米巴和溶组织内阿米巴的为45 kDa。
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