Burke E P, Sanders K M, Horowitz B
Department of Physiology, University of Nevada School of Medicine, Reno 89557.
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2370-4. doi: 10.1073/pnas.88.6.2370.
Molecular analyses of Na,K-ATPase abundance and alpha-subunit isoform distribution were performed to determine whether pump expression varies at different points through the thickness of the circular layer of colonic smooth muscle. The mRNA and polypeptides of Na,K-ATPase alpha 1 and beta subunits were twice as abundant in the submucosal region of the circular layer, which has previously been shown to generate large pump potentials. Sodium pump activity directly correlated with the relative abundance of the alpha 1 polypeptide. These data show that sodium pump expression varies in electrically dissimilar regions of the circular layer.
进行了钠钾ATP酶丰度和α亚基同工型分布的分子分析，以确定在结肠平滑肌环形层厚度的不同点处泵的表达是否存在差异。钠钾ATP酶α1和β亚基的mRNA及多肽在环形层的黏膜下层区域的丰度是其他区域的两倍，该区域先前已被证明可产生较大的泵电位。钠泵活性与α1多肽的相对丰度直接相关。这些数据表明，钠泵表达在环形层电特性不同的区域存在差异。
