Zhang Yi-Feng, Zhang Yan, Lu Cui-Yun, Cao Ding-Chen, Sun Xiao-Wen
Heilongjiang Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Harbin 150070, China.
Yi Chuan. 2008 May;30(5):613-9. doi: 10.3724/sp.j.1005.2008.00613.
Forty-seven microsatellite markers were selected to analyze the genomic DNA of 92 progenies derived from the recombinant inbred lines (RIL) of common carp, which came from the cross between Barbless carp and Hebao-cold tolerance red carp. The results showed that a total of 162 different alleles were found, and the number of alleles in each locus was 2 to 6. The DNA fragment length was 100 bp to 444 bp, and the number of mean valid alleles was 1.3069 to 4.2288. The value of heterozygosity was 0.2361 to 0.7677, and the mean polymorphism information content (PIC) was 0.5368. A GLM procedure was used to analyze the effects of these 47 microsatellites on body weight, length and height. Results uncovered HLJ695, HLJ716, HLJ739, HLJ759, HLJ774 and K16 had a significant impact on body weight, length and height, and HLJ776 had a significant impact on height. In addition, the genotypes of these correlative loci were determined.
选择47个微卫星标记对92个鲤重组自交系（RIL）子代的基因组DNA进行分析，这些子代来自无鳞鲤与荷包耐寒红鲤的杂交后代。结果表明，共发现162个不同等位基因，每个位点的等位基因数为2至6个。DNA片段长度为100 bp至444 bp，平均有效等位基因数为1.3069至4.2288。杂合度值为0.2361至0.7677，平均多态信息含量（PIC）为0.5368。使用广义线性模型（GLM）程序分析这47个微卫星对体重、体长和体高的影响。结果发现HLJ695、HLJ716、HLJ739、HLJ759、HLJ774和K16对体重、体长和体高有显著影响，HLJ776对体高有显著影响。此外，还确定了这些相关位点的基因型。
