Ma Ji-Min, Kuang You-Yi, Zheng Xian-Hu, Cao Ding-Chen, Lyu Wei-Hua, Chang Yu, Zhou Dan, DU Ke, Sun Xiao-Wen
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Dongwuxue Yanjiu. 2013 Aug;34(4):406-10. doi: 10.11813/j.issn.0254-5853.2013.4.0406.
In this study, 149 polymorphic markers were screened from 200 microsatellite markers. From a family of mirror carp, which included 107 individuals. All samples were analyzed for body correlation, and intermuscular bone number was tested using the General Linear Model (GLM) single marker regression. Determination of the threshold values by 10,000 permutation tests showed that eight markers had significant correlation (P<0.05), in which HLJ3086, HLJ3642 and HLJ3515 had very significant correlation with intermuscular bone number (P<0.01). In addition, the genotypes of the captured correlative loci were determined by Duncan's test using SPSS17.0 software. Markers were used to screen the protein and nucleotide database in the National Center for Biotechnology Information (NCBI). Blasting results showed that HLJ2891 was highly correlated (92%) with latrophilin-2-like and HLJ3515 was highly correlated (81%) with serine/threonine-protein kinase 32B-like of zebrafish. These functional markers and genotypes may provide an efficient basis for marker-assisted selection of intermuscular bone number in mirror carp.
在本研究中，从200个微卫星标记中筛选出149个多态性标记。样本来自一个包含107个个体的镜鲤家系。对所有样本进行体相关性分析，并使用一般线性模型（GLM）单标记回归测试肌间骨数量。通过10000次排列检验确定阈值，结果显示8个标记具有显著相关性（P<0.05），其中HLJ3086、HLJ3642和HLJ3515与肌间骨数量具有极显著相关性（P<0.01）。此外，使用SPSS17.0软件通过邓肯检验确定捕获的相关位点的基因型。利用这些标记在国家生物技术信息中心（NCBI）的蛋白质和核苷酸数据库中进行筛选。比对结果显示，HLJ2891与类latrophilin-2的相关性高达92%，HLJ3515与斑马鱼类丝氨酸/苏氨酸蛋白激酶32B-like的相关性高达81%。这些功能标记和基因型可为镜鲤肌间骨数量的分子标记辅助选择提供有效依据。
