Kumaki Yuichi, Oda Masaaki, Okano Masaki
Laboratory for Mammalian Epigenetic Studies, Center for Developmental Biology, RIKEN, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan.
Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W170-5. doi: 10.1093/nar/gkn294. Epub 2008 May 16.
Bisulfite sequencing, a standard method for DNA methylation profile analysis, is widely used in basic and clinical studies. This method is limited, however, by the time-consuming data analysis processes required to obtain accurate DNA methylation profiles from the raw sequence output of the DNA sequencer, and by the fact that quality checking of the results can be influenced by a researcher's bias. We have developed an interactive and easy-to-use web-based tool, QUMA (quantification tool for methylation analysis), for the bisulfite sequencing analysis of CpG methylation. QUMA includes most of the data-processing functions necessary for the analysis of bisulfite sequences. It also provides a platform for consistent quality control of the analysis. The QUMA web server is available at http://quma.cdb.riken.jp/.
亚硫酸氢盐测序是DNA甲基化谱分析的标准方法，广泛应用于基础研究和临床研究。然而，该方法存在局限性，从DNA测序仪的原始序列输出中获取准确的DNA甲基化谱需要耗时的数据处理过程，而且结果的质量检查可能会受到研究人员偏差的影响。我们开发了一种交互式且易于使用的基于网络的工具QUMA（甲基化分析定量工具），用于亚硫酸氢盐测序分析CpG甲基化。QUMA包含了亚硫酸氢盐序列分析所需的大部分数据处理功能。它还提供了一个用于分析的一致性质量控制平台。QUMA网络服务器可在http://quma.cdb.riken.jp/获取。
