将连续多个无痕基因缺失和无标记基因插入鼠疫耶尔森氏菌染色体的高效方法。
Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome.
作者信息
Sun Wei, Wang Shifeng, Curtiss Roy
机构信息
The Biodesign Institute, Center for Infectious Diseases and Vaccinology, Arizona State University, Tempe, AZ 85287-5401, USA.
出版信息
Appl Environ Microbiol. 2008 Jul;74(13):4241-5. doi: 10.1128/AEM.00940-08. Epub 2008 May 16.
Abstract
An efficient two-step recombination method for markerless gene deletion and insertion that can be used for repetitive genetic modification in Yersinia pestis was developed. The method combines lambda Red recombination and counterselective screening (sacB gene) and can be used for genetic modification of Y. pestis to construct live attenuated vaccines.
摘要
开发了一种高效的两步重组方法，用于无标记基因缺失和插入，可用于鼠疫耶尔森菌的重复基因修饰。该方法结合了λ Red重组和反选择筛选（sacB基因），可用于鼠疫耶尔森菌的基因修饰以构建减毒活疫苗。
相似文献
1
Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome.将连续多个无痕基因缺失和无标记基因插入鼠疫耶尔森氏菌染色体的高效方法。
Appl Environ Microbiol. 2008 Jul;74(13):4241-5. doi: 10.1128/AEM.00940-08. Epub 2008 May 16.
2
Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli.用于大肠杆菌中通用高效无标记DNA操作的两步红色介导重组。
Biotechniques. 2006 Feb;40(2):191-7. doi: 10.2144/000112096.
3
Sequence analysis of a Yersinia pestis insertion sequence associated with an unstable region of the chromosome.
Contrib Microbiol Immunol. 1995;13:294-8.
4
High-Efficiency, Two-Step Scarless-Markerless Genome Genetic Modification in Salmonella enterica.高效、两步、无痕、无标记基因遗传修饰沙门氏菌。
Curr Microbiol. 2016 Jun;72(6):700-6. doi: 10.1007/s00284-016-1002-3. Epub 2016 Feb 16.
5
CRISPR-Cas12a-Assisted Recombineering in Bacteria.细菌中CRISPR-Cas12a辅助的重组工程
Appl Environ Microbiol. 2017 Aug 17;83(17). doi: 10.1128/AEM.00947-17. Print 2017 Sep 1.
6
Proteins essential for expression of the Hms+ phenotype of Yersinia pestis.对鼠疫耶尔森菌Hms +表型表达至关重要的蛋白质。
Mol Microbiol. 1993 May;8(5):857-64. doi: 10.1111/j.1365-2958.1993.tb01632.x.
7
Calcium-regulated type III secretion of Yop proteins by an Escherichia coli hha mutant carrying a Yersinia pestis pCD1 virulence plasmid.携带鼠疫耶尔森菌pCD1毒力质粒的大肠杆菌hha突变体对Yop蛋白的钙调节III型分泌
Infect Immun. 2006 Feb;74(2):1381-6. doi: 10.1128/IAI.74.2.1381-1386.2006.
8
The 102-kilobase unstable region of Yersinia pestis comprises a high-pathogenicity island linked to a pigmentation segment which undergoes internal rearrangement.鼠疫耶尔森菌的102千碱基不稳定区域包含一个与色素沉着区段相连的高致病性岛，该色素沉着区段会发生内部重排。
J Bacteriol. 1998 May;180(9):2321-9. doi: 10.1128/JB.180.9.2321-2329.1998.
9
Genetic organization of the yersiniabactin biosynthetic region and construction of avirulent mutants in Yersinia pestis.鼠疫耶尔森氏菌中铁载体生物合成区域的基因组织及无毒突变体的构建
Infect Immun. 1997 May;65(5):1659-68. doi: 10.1128/iai.65.5.1659-1668.1997.
10
Differential gene regulation in Yersinia pestis versus Yersinia pseudotuberculosis: effects of hypoxia and potential role of a plasmid regulator.鼠疫耶尔森菌与假结核耶尔森菌的差异基因调控：缺氧的影响及一种质粒调节因子的潜在作用
Adv Exp Med Biol. 2007;603:131-44. doi: 10.1007/978-0-387-72124-8_11.
引用本文的文献
1
Immunogenicity and cross-protective efficacy induced by delayed attenuated with regulated length of lipopolysaccharide in mice.经调控长度脂多糖的减毒活疫苗在小鼠体内诱导的免疫原性和交叉保护效力。
Gut Microbes. 2024 Jan-Dec;16(1):2424983. doi: 10.1080/19490976.2024.2424983. Epub 2024 Nov 11.
2
Recombinant Attenuated Vaccine Displaying Regulated Lysis to Confer Biological Containment and Protect Catfish against Edwardsiellosis.展示调控裂解以实现生物遏制并保护鲶鱼免受爱德华氏菌病侵害的重组减毒疫苗。
Vaccines (Basel). 2023 Sep 9;11(9):1470. doi: 10.3390/vaccines11091470.
3
Recombineering in Non-Model Bacteria.非模式细菌中的重组。
Curr Protoc. 2022 Dec;2(12):e605. doi: 10.1002/cpz1.605.
4
Dissecting Locus Regulation in Yersinia pestis.解析鼠疫耶尔森氏菌中的基因调控区。
J Bacteriol. 2021 Sep 8;203(19):e0023721. doi: 10.1128/JB.00237-21.
5
barCoder: a tool to generate unique, orthogonal genetic tags for qPCR detection.barCoder：一种用于生成 qPCR 检测中独特、正交遗传标签的工具。
BMC Bioinformatics. 2021 Mar 1;22(1):98. doi: 10.1186/s12859-021-04019-5.
6
Bacterial Genetic Engineering by Means of Recombineering for Reverse Genetics.通过重组工程进行细菌遗传工程以实现反向遗传学
Front Microbiol. 2020 Sep 11;11:548410. doi: 10.3389/fmicb.2020.548410. eCollection 2020.
7
Induction of Protective Antiplague Immune Responses by Self-Adjuvanting Bionanoparticles Derived from Engineered Yersinia pestis.工程化鼠疫耶尔森菌衍生的自佐剂化生物纳米颗粒诱导保护性抗鼠疫免疫应答。
Infect Immun. 2020 Apr 20;88(5). doi: 10.1128/IAI.00081-20.
8
Protection and Safety Evaluation of Live Constructions Derived from the Pgm and pPCP1 Strain.源自Pgm和pPCP1菌株的活菌制剂的保护作用及安全性评估
Vaccines (Basel). 2020 Feb 21;8(1):95. doi: 10.3390/vaccines8010095.
9
A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis.CRISPR 辅助的非同源末端连接策略可有效编辑结核分枝杆菌基因组。
mBio. 2020 Jan 28;11(1):e02364-19. doi: 10.1128/mBio.02364-19.
10
Engineering the PduT shell protein to modify the permeability of the 1,2-propanediol microcompartment of .工程化 PduT 外壳蛋白以改变. 1,2-丙二醇微隔间的通透性。
Microbiology (Reading). 2019 Dec;165(12):1355-1364. doi: 10.1099/mic.0.000872.
本文引用的文献
1
A putative DNA adenine methyltransferase is involved in Yersinia pseudotuberculosis pathogenicity.一种假定的DNA腺嘌呤甲基转移酶参与了假结核耶尔森菌的致病性。
Microbiology (Reading). 2007 Aug;153(Pt 8):2426-2434. doi: 10.1099/mic.0.2007/005736-0.
2
RovA, a global regulator of Yersinia pestis, specifically required for bubonic plague.RovA是鼠疫耶尔森菌的一种全局调节因子，是腺鼠疫特别需要的。
Proc Natl Acad Sci U S A. 2006 Sep 5;103(36):13514-9. doi: 10.1073/pnas.0603456103. Epub 2006 Aug 28.
3
A dam mutant of Yersinia pestis is attenuated and induces protection against plague.鼠疫耶尔森菌的dam突变体减毒并诱导抗鼠疫保护作用。
FEMS Microbiol Lett. 2005 Nov 15;252(2):251-6. doi: 10.1016/j.femsle.2005.09.001. Epub 2005 Sep 15.
4
Rapid method for the construction of Salmonella enterica Serovar Typhimurium vaccine carrier strains.肠炎沙门氏菌鼠伤寒血清型疫苗载体菌株的快速构建方法。
Infect Immun. 2005 Mar;73(3):1598-605. doi: 10.1128/IAI.73.3.1598-1605.2005.
5
A rapid and simple method for inactivating chromosomal genes in Yersinia.一种快速简便的使耶尔森氏菌染色体基因失活的方法。
FEMS Immunol Med Microbiol. 2003 Sep 22;38(2):113-6. doi: 10.1016/S0928-8244(03)00181-0.
6
Genome sequence of Yersinia pestis KIM.鼠疫杆菌KIM株的基因组序列。
J Bacteriol. 2002 Aug;184(16):4601-11. doi: 10.1128/JB.184.16.4601-4611.2002.
7
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.利用PCR产物一步灭活大肠杆菌K-12中的染色体基因。
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5. doi: 10.1073/pnas.120163297.
8
PCR-mediated gene replacement in Escherichia coli.大肠杆菌中PCR介导的基因置换
Gene. 2000 Apr 4;246(1-2):321-30. doi: 10.1016/s0378-1119(00)00071-8.
9
Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.利用噬菌体λ重组功能促进大肠杆菌中的基因替换。
J Bacteriol. 1998 Apr;180(8):2063-71. doi: 10.1128/JB.180.8.2063-2071.1998.
10
Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression.改进的等位基因交换载体及其在分析987P菌毛基因表达中的应用。
Gene. 1998 Jan 30;207(2):149-57. doi: 10.1016/s0378-1119(97)00619-7.
Zotero 插件