利用噬菌体λ重组功能促进大肠杆菌中的基因替换。
Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.
作者信息
Murphy K C
机构信息
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655, USA.
出版信息
J Bacteriol. 1998 Apr;180(8):2063-71. doi: 10.1128/JB.180.8.2063-2071.1998.
Abstract
Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment. The deltarecBCD::Plac-red kan replacement allele can be P1 transduced to other E. coli strains, making the hyper-Rec phenotype easily transferable.
摘要
用噬菌体λ的Red功能替代大肠杆菌的RecBCD功能，可产生一种菌株，其染色体与短线性DNA片段的重组率大幅提高。该重组率至少比recBC sbcBC或recD菌株高70倍。通过用PCR产生的DNA片段进行基因替换，突出了该系统的价值。deltarecBCD::Plac-red kan替换等位基因可通过P1转导至其他大肠杆菌菌株，使超Rec表型易于转移。
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