Bellini Maria Helena, Coutinho Enia Lúcia, Courrol Lilia Coronato, Rodrigues de Oliveira Silva Flávia, Vieira Júnior Nilson Dias, Schor Nestor
Disciplina de Nefrologia, Departamento de Medicina, UNIFESP, São Paulo, SP, Brazil.
J Fluoresc. 2008 Nov;18(6):1163-8. doi: 10.1007/s10895-008-0368-4. Epub 2008 May 17.
Protoporphyrin IX (PpIX) is a porphyrin derivative that is accumulated in cancerous tissue in consequence of the tumor-specific metabolic alterations. The aim of this study was to evaluate the accumulation of PpIX in mice bearing renal cell carcinoma by spectroscopy analysis. A total of 24 male Balb/c mice, 6 weeks old, were divided into six groups: Normal (without inoculation of tumor cells) and 4, 8, 13, 16, and 20 days after inoculation of tumor cells. The orthotopic tumor model of renal cancer was used. Murine renal cell carcinoma (Renca cells) were inoculated into the subcapsular space of the kidney. Normal and tumor-bearing kidneys in different progression stages were removed and analyzed by ex-vivo spectroscopy and by microscopy, for tumor histometric analysis. Emission spectra were obtained by exciting the samples at 405 nm. Significant differences between normal and tumor-bearing kidneys in autofluorescence shape occurred in the 600-700 nm spectral region. A good correlation was found between emission band intensity at 635 nm and the tumor area.
原卟啉IX（PpIX）是一种卟啉衍生物，由于肿瘤特异性代谢改变而在癌组织中蓄积。本研究的目的是通过光谱分析评估PpIX在荷肾细胞癌小鼠中的蓄积情况。将总共24只6周龄的雄性Balb/c小鼠分为六组：正常组（未接种肿瘤细胞）以及接种肿瘤细胞后4、8、13、16和20天的组。采用肾癌原位肿瘤模型。将小鼠肾细胞癌（Renca细胞）接种到肾被膜下间隙。切除处于不同进展阶段的正常和荷瘤肾脏，并通过离体光谱和显微镜进行分析，以进行肿瘤组织计量学分析。通过在405nm激发样品获得发射光谱。在600 - 700nm光谱区域，正常肾脏和荷瘤肾脏的自发荧光形状存在显著差异。在635nm处的发射带强度与肿瘤面积之间发现了良好的相关性。
