Fukuda Y, Imoto M, Koyama Y, Miyazawa Y, Nakano I, Hattori M, Urano F, Kodama S, Iwata K, Hayakawa T
Second Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Gastroenterol Jpn. 1991 Feb;26(1):37-41. doi: 10.1007/BF02779506.
The localization of tissue inhibitor of metalloproteinases (TIMP) in normal and pathological livers was examined by immunohistochemistry using monoclonal antibodies at the light microscopic level. In normal liver, immunoreactive TIMP was detected in smooth muscle cells and endothelial cells of blood vessels, fibroblasts, bile duct cells and Kupffer cells, indicating that TIMP is likely to be a general element of the liver. Immunoreactivity was observed in newly-formed blood vessels, proliferating bile ductules, and fibroblasts in the expanded portal area and fibrous septa of chronic active hepatitis and cirrhosis. TIMP was strongly stained in the capsule of hepatocellular carcinoma. The intensity of the immunoreaction in the capsule was generally greater than that in cirrhotic liver apart from the tumor mass. In three of five cases with hepatocellular carcinoma, endothelial walls in contact with tumor cells were positive.
采用单克隆抗体免疫组化法在光镜水平检测金属蛋白酶组织抑制剂（TIMP）在正常肝脏和病理肝脏中的定位。在正常肝脏中，免疫反应性TIMP在血管平滑肌细胞、内皮细胞、成纤维细胞、胆管细胞和库普弗细胞中被检测到，这表明TIMP可能是肝脏的一种普遍成分。在慢性活动性肝炎和肝硬化的扩大门静脉区和纤维间隔中的新生血管、增生的胆小管和成纤维细胞中观察到免疫反应性。TIMP在肝细胞癌的包膜中被强烈染色。除肿瘤块外，包膜中的免疫反应强度一般大于肝硬化肝脏中的强度。在五例肝细胞癌病例中的三例中，与肿瘤细胞接触的内皮壁呈阳性。
