Kalebic T, Garbisa S, Glaser B, Liotta L A
Science. 1983 Jul 15;221(4607):281-3. doi: 10.1126/science.6190230.
One of the first steps in neovascularization is dissolution of the basement membrane at the point of endothelial outgrowth. An assay was developed to determine whether basement membrane collagens (types IV and V) are degraded by endothelial cells migrating toward a chemotactic stimulus. Fetal bovine endothelial cells were placed on one side of a filter containing the collagen substrate, and a chemoattractant derived from retinal extracts was placed on the opposite side. Degradation of both type IV and type V collagens was observed when the retinal factor was placed on the side of the filter opposite the endothelial cells. Metalloproteinases that cleaved type IV and type V collagens could be extracted from the endothelial cells with detergents. Such endothelial cell-associated (possibly membrane-bound) proteinases may locally disrupt the basement membrane and facilitate the outgrowth of capillary sprouts toward the angiogenic stimulus.
血管生成的首要步骤之一是在内皮细胞生长点处基底膜的溶解。人们开发了一种检测方法，以确定基底膜胶原蛋白（IV型和V型）是否会被向内皮细胞趋化刺激迁移的内皮细胞降解。将胎牛内皮细胞置于含有胶原蛋白底物的滤膜一侧，将源自视网膜提取物的趋化因子置于另一侧。当视网膜因子置于滤膜上与内皮细胞相对的一侧时，可观察到IV型和V型胶原蛋白均被降解。用去污剂可从内皮细胞中提取能切割IV型和V型胶原蛋白的金属蛋白酶。这种与内皮细胞相关的（可能是膜结合的）蛋白酶可能会局部破坏基底膜，并促进毛细血管芽向血管生成刺激物生长。
