Zhang Li, Huang Cheng Zhi, Li Yuan Fang, Xiao Sai Jin, Xie Jian Ping
College of Chemistry and Chemical Engineering, MOE Key Laboratory of Luminescence and Real-Time Analysis, CQKL-LRTA, Southwest University, Chongqing, China.
J Phys Chem B. 2008 Jun 12;112(23):7120-2. doi: 10.1021/jp800092r. Epub 2008 May 20.
A detection method for DNA sequence-specificity in a homogeneous medium is presented with multiwalled carbon nanotubes (MWCNTs) as optical probes on the basis of the measurements of light scattering signals. ssDNA can prevent MWCNTs from coagulation in electrolyte solution while dsDNA cannot, displaying different light scattering signals. With the light scattering signals, target DNA in the range of 8.6-86.4 nM could be detected and one base pair mismatch could be discriminated. The sequence specificity for the present method has been identified with PCR products.
基于光散射信号的测量，提出了一种以多壁碳纳米管（MWCNTs）作为光学探针在均相介质中检测DNA序列特异性的方法。单链DNA（ssDNA）可防止MWCNTs在电解质溶液中凝聚，而双链DNA（dsDNA）则不能，二者呈现出不同的光散射信号。利用这些光散射信号，可检测8.6 - 86.4 nM范围内的目标DNA，并且能够区分一个碱基对的错配。该方法对PCR产物的序列特异性已得到验证。
