Cardona F, Túnez I, Tasset I, Montilla P, Collantes E, Tinahones F J
Endocrinology and Nutrition Service, Virgen de la Victoria University Hospital, 29010 Malaga, Spain.
Eur J Clin Invest. 2008 Jul;38(7):510-5. doi: 10.1111/j.1365-2362.2008.01959.x. Epub 2008 May 16.
Patients with the metabolic syndrome have greater levels of oxidative stress. However, as the response of markers of this stress to a fat overload is unknown, we evaluated certain markers of oxidative stress in these patients.
The study population comprised 93 subjects (70 men and 23 women): 13 healthy people (controls) with a mean age of 48.81 +/- 9.01 years and 80 patients with the metabolic syndrome (mean age, 43.25 +/- 11.55 years), according to the Adult Treatment Panel III criteria. All the participants were given a 60 g fat overload (Supracal). Three hours later the following biomarkers of oxidative stress were measured: lipid peroxidation products, protein carbonyl groups, reduced glutathione, glutathione peroxidase (GSH-Px), catalase, superoxide dismutase, glutathione reductase (GSH-Road) and glutathione S-transferase. The levels of oxidized glutathione (GSSG) were calculated.
Compared with the controls, the patients showed greater baseline oxidative stress, higher levels of lipid peroxidation products and oxidized glutathione, and lower levels of reduced glutathione, glutathione peroxidase activity, glutathione reductase and glutathione transferase. This stress was more intense after the subjects received a fat overload, more so in the patients who experienced a greater reduction in GSHpx and GSHrd antioxidant activity and a greater increase in the levels of carbonylated proteins and lipoperoxides than the controls.
Patients with the metabolic syndrome have greater oxidative stress than healthy people. The variation in markers of this stress after a fat overload was even more pronounced in the patients.
代谢综合征患者具有更高水平的氧化应激。然而，由于这种应激标志物对脂肪超载的反应尚不清楚，我们评估了这些患者的某些氧化应激标志物。
研究人群包括93名受试者（70名男性和23名女性）：13名健康人（对照组），平均年龄48.81±9.01岁，80名代谢综合征患者（平均年龄43.25±11.55岁），符合成人治疗小组III标准。所有参与者均接受60克脂肪超载（Supracal）。三小时后，测量以下氧化应激生物标志物：脂质过氧化产物、蛋白质羰基、还原型谷胱甘肽、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶、超氧化物歧化酶、谷胱甘肽还原酶（GSH-Road）和谷胱甘肽S-转移酶。计算氧化型谷胱甘肽（GSSG）水平。
与对照组相比，患者表现出更高的基线氧化应激、更高水平的脂质过氧化产物和氧化型谷胱甘肽，以及更低水平的还原型谷胱甘肽、谷胱甘肽过氧化物酶活性、谷胱甘肽还原酶和谷胱甘肽转移酶。在受试者接受脂肪超载后，这种应激更为强烈，尤其是那些谷胱甘肽过氧化物酶和谷胱甘肽还原酶抗氧化活性降低幅度更大、羰基化蛋白质和脂质过氧化物水平升高幅度比对照组更大的患者。
代谢综合征患者比健康人具有更高的氧化应激。在脂肪超载后，这种应激标志物的变化在患者中更为明显。
