Sakhaei Fariba, Keshvari Mahtab, Asgary Sedigheh, Salehizadeh Leila, Rastqar Ali, Samsam-Shariat Seyyed Ziaedin
Isfahan Pharmaceutical Sciences Research Center AND Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
ARYA Atheroscler. 2020 Mar;16(2):94-101. doi: 10.22122/arya.v16i2.1813.
This study examined the relationship between serum glutathione peroxidase 1 (GPx-1) activity and endothelial dysfunction in the subjects with and without metabolic syndrome (MetS).
This case-control study was conducted on 76 subjects, 38 were patients with MetS and 38 were without MetS. The demographic, clinical, and laboratory features of the subjects were measured and then compared. The MetS was diagnosed according to the definitions of the National Cholesterol Education Program (NCEP) and International Diabetes Federation (IDF). Serum GPx-1 activity was measured by standard methods. Endothelial dysfunction was assessed with flow-mediated dilation (FMD) technique.
In case-control study of 76 subjects, all of MetS risk factors including abdominal obesity, triglyceride (TG), low serum level of high-density lipoprotein cholesterol (HDL-C), hypertension (HTN), and fasting plasma glucose (FPG) were significantly higher than healthy individuals (P < 0.050). FMD was significantly lower than normal subjects (P < 0.050). Serum GP-1 activity was significantly lower in patients with MetS compared to normal subjects (21.7 ± 13.5 vs. 79.0 ± 38.6, respectively) (P = 0.001). The value of GPx-1 was significantly correlated with diastolic blood pressure (DBP) (r = -0.249, P = 0.040), C-reactive protein (CRP) (r = -0.409, P = 0.014), and FMD (r = 0.293, P = 0.050) in patients with MetS. The results of logistic regression showed that a unite increase in CRP (mg/dl), FMD (%), and endothelin-1 (ET-1) (pg/ml) and a unit decrease in GPx significantly increased the odds ratio (OR) of MetS; after adjusting for age and sex the results remained significant except for FMD (P < 0.050).
Endothelial dysfunction is related to serum GPx-1 activity in patients with MetS. GPX-1 activity is associated with risk of cardiovascular diseases (CVDs) and peripheral vascular diseases (PVDs) in patients with MetS.
本研究调查了患有和未患有代谢综合征（MetS）的受试者血清谷胱甘肽过氧化物酶1（GPx - 1）活性与内皮功能障碍之间的关系。
本病例对照研究纳入了76名受试者，其中38名患有MetS，38名未患有MetS。测量并比较了受试者的人口统计学、临床和实验室特征。根据美国国家胆固醇教育计划（NCEP）和国际糖尿病联盟（IDF）的定义诊断MetS。采用标准方法测量血清GPx - 1活性。用血流介导的血管舒张（FMD）技术评估内皮功能障碍。
在对76名受试者的病例对照研究中，所有MetS危险因素，包括腹型肥胖、甘油三酯（TG）、血清高密度脂蛋白胆固醇（HDL - C）水平低、高血压（HTN）和空腹血糖（FPG）均显著高于健康个体（P < 0.050）。FMD显著低于正常受试者（P < 0.050）。与正常受试者相比，MetS患者的血清GP - 1活性显著降低（分别为21.7±13.5和79.0±38.6）（P = 0.001）。在患有MetS的患者中，GPx - 1的值与舒张压（DBP）（r = - 0.249，P = 0.040）、C反应蛋白（CRP）（r = - 0.409，P = 0.014）和FMD（r = 0.293，P = 0.050）显著相关。逻辑回归结果显示，CRP（mg/dl）、FMD（%）和内皮素 - 1（ET - 1）（pg/ml）每增加一个单位，GPx每降低一个单位，MetS的比值比（OR）显著增加；在调整年龄和性别后，除FMD外，结果仍然显著（P < 0.050）。
MetS患者的内皮功能障碍与血清GPx - 1活性有关。GPX - 1活性与MetS患者的心血管疾病（CVDs）和外周血管疾病（PVDs）风险相关。
