一种检测磷脂模型膜内单线态氧的新型荧光方法。
A new fluorescence method to detect singlet oxygen inside phospholipid model membranes.
作者信息
Wozniak M, Tanfani F, Bertoli E, Zolese G, Antosiewicz J
机构信息
Department of Biochemistry, Medical Academic School, Gdansk, Poland.
出版信息
Biochim Biophys Acta. 1991 Feb 26;1082(1):94-100. doi: 10.1016/0005-2760(91)90304-z.
A fluorescence method for detecting singlet oxygen (1O2) in model membranes is proposed. 1O2 was generated by hydrogen peroxide/sodium hypochlorite system. 1,3-Diphenylisobenzofuran (DPBF), a specific 1O2 trap, dissolved in organic solvents gives a strong fluorescence spectrum when excited at 410 nm. A similar spectrum, with a maximum at 455 nm, is obtained when DPBF is incorporated in unilamellar dipalmitoylphosphatidylcholine liposomes. The intensity of fluorescence spectrum decreases when DPBF-labeled liposomes are exposed to singlet oxygen. This decrease is sensitive to 1O2 traps and quenchers like tryptophan and sodium azide, to lipid membrane fluidity and to the concentration of sodium hypochlorite and hydrogen peroxide.
提出了一种用于检测模型膜中单线态氧（¹O₂）的荧光方法。¹O₂由过氧化氢/次氯酸钠体系产生。1,3-二苯基异苯并呋喃（DPBF）是一种特定的¹O₂捕获剂，溶解于有机溶剂中时，在410 nm激发下会产生强烈的荧光光谱。当DPBF掺入单层二棕榈酰磷脂酰胆碱脂质体中时，会获得一个类似的光谱，其最大值在455 nm处。当用DPBF标记的脂质体暴露于单线态氧时，荧光光谱强度会降低。这种降低对¹O₂捕获剂和猝灭剂（如色氨酸和叠氮化钠）、脂质膜流动性以及次氯酸钠和过氧化氢的浓度敏感。
Zotero 插件