Dalpathado Dilusha S, Desaire Heather
Department of Chemistry, University of Kansas, Lawrence, KS 66045, USA.
Analyst. 2008 Jun;133(6):731-8. doi: 10.1039/b713816d. Epub 2008 Mar 19.
Glycosylation is one of the most important post-translational modifications found in nature. Identifying and characterizing glycans is an important step in correlating glycosylation structure to the glycan's function, both in normal glycoproteins and those that are modified in a disease state. Glycans on a protein can be characterized by a variety of methods. This review focuses on the mass spectral analysis of glycopeptides, after subjecting the glycoprotein to proteolysis. This analytical approach is useful in characterizing glycan heterogeneity and correlating glycan compositions to their attachment sites on the protein. The information obtained from this approach can serve as the foundation for understanding how glycan compositions affect protein function, in both normal and aberrant glycoproteins.
糖基化是自然界中最重要的翻译后修饰之一。在正常糖蛋白和疾病状态下修饰的糖蛋白中，识别和表征聚糖是将糖基化结构与聚糖功能相关联的重要一步。蛋白质上的聚糖可以通过多种方法进行表征。本综述重点关注糖蛋白经蛋白酶解后糖肽的质谱分析。这种分析方法有助于表征聚糖的异质性，并将聚糖组成与其在蛋白质上的附着位点相关联。从这种方法获得的信息可作为理解聚糖组成如何影响正常和异常糖蛋白中蛋白质功能的基础。
