Lerman M I, Pilipenko N N, Ygarova T Y, Sokolova E S, Vinnizky L I, Phishkova Z P
Cancer Res. 1976 Sep;36(9 pt.1):2995-3000.
Using crude cell extracts from rapidly growing animal and human tumors, we found that (a) the addition of homologous transfer RNA (tRNA) to these extracts stimulated polypeptide synthesis two-to threefold, while addition of heterologous tRNA did not have a similar effect; (b) addition of homologous as well as heterologous ribosomal RNA was also stimulatory; and (c) both stimulatory effects were additive. The possibility that the effect of homologous tRNA could be mediated by contaminating material (such as the "translational control" RNA) seems to be rulted out by experiments with highly purified tRNA preparations, which did not contain even traces of 18 S, 7 S, 5 S, and smaller than 4S RNA's. Control experiments showed that no loss of tRNA occurred either during preparation of the cell extracts or under the conditions of in vitro protein synthesis. The results obtained suggest possible occurrence of a deficiency in specific isoaccepting tRNA's in rapidly growing solid tumors.
利用快速生长的动物和人类肿瘤的粗细胞提取物，我们发现：（a）向这些提取物中添加同源转移RNA（tRNA）可使多肽合成增加两到三倍，而添加异源tRNA则没有类似效果；（b）添加同源以及异源核糖体RNA也具有刺激作用；（c）两种刺激作用是相加的。同源tRNA的作用可能由污染物（如“翻译控制”RNA）介导，这一可能性似乎已被使用高度纯化的tRNA制剂的实验排除，这些制剂甚至不含痕量的18 S、7 S、5 S和小于4S的RNA。对照实验表明，在制备细胞提取物的过程中或体外蛋白质合成的条件下，tRNA均未发生损失。所获得的结果表明，在快速生长的实体瘤中可能存在特定同功tRNA的缺乏。
