Bcl-xL在人类结肠癌中的意义。
Significance of Bcl-xL in human colon carcinoma.
作者信息
Zhang You-Li, Pang Li-Qun, Wu Ying, Wang Xiao-Yan, Wang Chong-Qiang, Fan Yu
机构信息
Department of Chemotherapy, Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002, Jiangsu Province, China.
出版信息
World J Gastroenterol. 2008 May 21;14(19):3069-73. doi: 10.3748/wjg.14.3069.
AIM
To investigate the clinical significance of Bcl-xL gene in the pathogenesis of human colon carcinoma.
METHODS
Fifty-six pair tissue samples from patients with colon cancer were collected, and protein level of the Bcl-xL gene was measured by immunohistochemistry method. The correlation of Bcl-xL expression with clinical index was evaluated. After human colon cancer cell line HT29 was transfected with Bcl-xL small interfering RNA (siRNA), the anchorage-independent growth of cancer cells was detected by colony formation in soft agar and invasion ability of cancer cells was determined by a transwell model.
RESULTS
The Bcl-xL expression was higher in cancerous tissue samples than in normal tissue samples (38.78 +/-11.36 vs 0.89 +/- 0.35, P < 0.001), and was associated with the pathological grade, lymph node metastasis and Duke's stage of colorectal carcinoma. Transfection with Bcl-xL siRNA inhibited the colony formation and invasion ability of human colon cancer cell line HT29 in vitro.
CONCLUSION
Bcl-xL gene plays an important role in carcinogenesis of human colorectal carcinoma and is associated with malignant biological behaviors of human colorectal carcinoma.
目的
探讨Bcl-xL基因在人类结肠癌发病机制中的临床意义。
方法
收集56对结肠癌患者的组织样本，采用免疫组织化学方法检测Bcl-xL基因的蛋白水平。评估Bcl-xL表达与临床指标的相关性。用Bcl-xL小干扰RNA（siRNA）转染人结肠癌细胞系HT29后，通过软琼脂集落形成检测癌细胞的非锚定依赖性生长，并通过Transwell模型测定癌细胞的侵袭能力。
结果
癌组织样本中Bcl-xL表达高于正常组织样本（38.78±11.36 vs 0.89±0.35，P<0.001），且与结直肠癌的病理分级、淋巴结转移及Duke分期相关。用Bcl-xL siRNA转染可抑制人结肠癌细胞系HT29在体外的集落形成和侵袭能力。
结论
Bcl-xL基因在人类结直肠癌的发生中起重要作用，并与人类结直肠癌的恶性生物学行为相关。
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