Ichimura H, Yamasaki M, Tamura I, Katsumoto T, Sawada M, Kurimura O, Furuyama J, Kurimura T
Institute of Clinical Research, Kure National Hospital, Japan.
Cancer. 1991 May 1;67(9):2327-32. doi: 10.1002/1097-0142(19910501)67:9<2327::aid-cncr2820670919>3.0.co;2-f.
A new cell line, designated TC-YIK, was established from YIK-1 tumor cells, derived from argyrophil small cell carcinoma (ASCC) of the uterine cervix, and serially heterotransplanted into nude mice, integrating human papillomavirus type 16 (HPV16) DNA. The population doubling time of TC-YIK was approximately 21.6 hours at the 119th subculture. Subcutaneous injection of 1 x 10(8) TC-YIK cells into nude mice yielded a solid tumor. The cytologic appearance of TC-YIK was similar to that of YIK-1. The TC-YIK cells contained argyrophil granules and neurosecretory granules in the cytoplasm and showed positive immunohistochemical staining for neuron-specific enolase, serotonin, and chromogranin. Thus, TC-YIK retained the histochemical characteristics of ASCC. The TC-YIK cells contained HPV16 DNA in a multiple-copy integrated form and actively transcribed the integrated HPV16 genome. Amplification of the c-myc oncogene was observed in the TC-YIK cells. These data suggest that TC-YIK is a useful in vitro experimental model of ASCC and that HPV16 and c-myc may play some role in the genesis of this malignant tumor and/or maintenance of the transformed TC-YIK phenotype.
一种新的细胞系，命名为TC-YIK，是从YIK-1肿瘤细胞建立的，YIK-1肿瘤细胞源自子宫颈嗜银性小细胞癌（ASCC），并连续异种移植到裸鼠体内，整合了16型人乳头瘤病毒（HPV16）DNA。在第119代传代培养时，TC-YIK的群体倍增时间约为21.6小时。将1×10⁸个TC-YIK细胞皮下注射到裸鼠体内可形成实体瘤。TC-YIK的细胞学表现与YIK-1相似。TC-YIK细胞的细胞质中含有嗜银颗粒和神经分泌颗粒，并且神经元特异性烯醇化酶、血清素和嗜铬粒蛋白的免疫组织化学染色呈阳性。因此，TC-YIK保留了ASCC的组织化学特征。TC-YIK细胞含有多拷贝整合形式的HPV16 DNA，并能积极转录整合的HPV16基因组。在TC-YIK细胞中观察到c-myc癌基因的扩增。这些数据表明，TC-YIK是一种有用的ASCC体外实验模型，并且HPV16和c-myc可能在这种恶性肿瘤的发生和/或转化的TC-YIK表型的维持中发挥某些作用。
