O'Brien Margaret, Morrison John J, Smith Terry J
National Centre for Biomedical and Engineering Science, National University of Ireland Galway, Galway, Ireland.
Reprod Sci. 2008 Apr;15(4):382-93. doi: 10.1177/1933719108316179.
The regulatory mechanisms underlying myometrial smooth muscle contractility during labor are poorly understood. The authors therefore investigated the transcriptional profile of the changes that occur in the human myometrium at term pregnancy when compared with that at labor. Microarray technology was used to identify differentially expressed genes in human myometrium at labor. Real-time fluorescence reversetranscriptase polymerase chain reaction (RT-PCR) was subsequently performed to verify the microarray data. Semiquantitative RT-PCR, Western blotting, and microscopy methodologies were also used. Certain novel genes were found to be upregulated in human myometrium at labor. Of these, PSCDBP, TLR2, TWIST1 , FLJ35382, andRGS12 have not been previously characterized or identified in human myometrium. EDNRB is the other novel labor-associated gene whose reported expression is also upregulated at labor. All 6 genes were expressed on human myometrial smooth muscle cells. These novel upregulated genes are involved in multiple pathways that may be associated with a variety of cellular processes including inflammation, transcriptional regulation, and intracellular signaling.
分娩期间子宫肌层平滑肌收缩性的调控机制目前仍知之甚少。因此，作者研究了足月妊娠时与分娩时相比，人子宫肌层发生的变化的转录谱。利用微阵列技术鉴定分娩时人子宫肌层中差异表达的基因。随后进行实时荧光逆转录聚合酶链反应（RT-PCR）以验证微阵列数据。还使用了半定量RT-PCR、蛋白质印迹法和显微镜方法。发现某些新基因在分娩时人子宫肌层中上调。其中，PSCDBP、TLR2、TWIST1、FLJ35382和RGS12此前尚未在人子宫肌层中得到表征或鉴定。EDNRB是另一个与分娩相关的新基因，其报道的表达在分娩时也上调。所有6个基因均在人子宫肌层平滑肌细胞上表达。这些新的上调基因参与多种途径，可能与包括炎症、转录调控和细胞内信号传导在内的多种细胞过程相关。
