Esplin M Sean, Peltier M R, Hamblin S, Smith S, Fausett M Bardett, Dildy G A, Branch D Ware, Silver R M, Adashi E Y
Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Utah Health Science Center, Salt Lake City, 84132, USA.
Placenta. 2005 Sep-Oct;26(8-9):661-71. doi: 10.1016/j.placenta.2004.09.012. Epub 2004 Dec 8.
Microarray analysis was used to characterize the labor-selective transcriptome of the human myometrium during labor. One highly up-regulated transcript, monocyte chemotactic protein-1 (MCP-1), was further characterized.
Expression of MCP-1 was evaluated in the myometrium, the placenta, the gestational membranes (GM) and the amniotic fluid (AF) by real time RT-PCR, Northern blot analysis and ELISA. The level of immunoreactive (IR) MCP-1 content of primary myometrial cultures treated with inflammatory cytokines was quantified by ELISA.
Up-regulation of the myometrial MCP-1 transcript in term laboring patients was demonstrated by microarray and confirmed by real time (RT)-PCR and Northern blot analysis. Increased MCP-1 transcripts were demonstrated in GM during term labor. The IR content of myometrial MCP-1 was increased during term labor and in the AF from patients experiencing preterm delivery. Levels of IR MCP-1 increased in myometrial cultures in response to interleukin 1-beta.
The expression of myometrial MCP-1 was significantly increased during term labor and was similarly increased in vitro in response to interleukin 1-beta, a pro-inflammatory substance known to play a role in preterm birth. The increased IR content of MCP-1 within the AF preceding preterm delivery may render this protein a useful predictor of preterm birth.
采用微阵列分析来表征分娩期间人子宫肌层的分娩选择性转录组。对一种高度上调的转录物单核细胞趋化蛋白-1（MCP-1）进行了进一步表征。
通过实时逆转录聚合酶链反应（RT-PCR）、Northern印迹分析和酶联免疫吸附测定（ELISA）评估MCP-1在子宫肌层、胎盘、胎膜（GM）和羊水（AF）中的表达。采用ELISA对用炎性细胞因子处理的原代子宫肌层培养物中免疫反应性（IR）MCP-1的含量水平进行定量。
微阵列显示足月分娩患者子宫肌层MCP-1转录物上调，并经实时（RT）-PCR和Northern印迹分析证实。足月分娩期间GM中MCP-1转录物增加。足月分娩时子宫肌层MCP-1的IR含量增加，早产患者羊水中的MCP-1也增加。子宫肌层培养物中IR MCP-1水平因白细胞介素1-β而升高。
足月分娩期间子宫肌层MCP-1的表达显著增加，体外对白细胞介素1-β（一种已知在早产中起作用的促炎物质）的反应也同样增加。早产前羊水中MCP-1的IR含量增加可能使该蛋白成为早产的有用预测指标。
