Brevet Marie, Ahidouch Ahmed, Sevestre Henri, Merviel Philippe, El Hiani Yassine, Robbe Micheline, Ouadid-Ahidouch Halima
Laboratoire de Physiologie Cellulaire et Moléculaire, EA 2086, Faculté des Sciences, Amiens, France.
Histol Histopathol. 2008 Aug;23(8):965-72. doi: 10.14670/HH-23.965.
Potassium (K+) channels contribute to the regulation of cell proliferation and apoptosis and are also involved in tumor generation and malignant growth. Using immunohistochemical analysis, we investigated the expression of four K+ channels GIRK1 (G-Protein Inwardly Rectifying Potassium Channel 1), Ca2+-activated K channel (K Ca 1.1), voltage activated K+ channels (KV 1.1 and KV 1.3) and of the anti-apoptotic protein Bcl2 in normal and cancerous breast tissues and compared their expression with clinicopathological data. GIRK1 was overexpressed in carcinomatous tissues. In contrast, K V 1.1 and K V 1.3 were less expressed in cancerous tissue. The expression of Bcl-2 was similar in both tissues. As to the clinicopathological data, a correlation between K Ca 1.1 channel and estrogen receptor (ER) expression was observed. GIRK1 was overexpressed in breast carcinoma suggesting its involvement in proliferation and oncogenesis and its possible use as a putative pharmaceutical target. The correlation between K Ca 1.1 channel and ER suggests the involvement of this channel in proliferation. The loss of expression of the two channels K V 1.1 and K V 1.3 may correspond to their role in apoptosis.
钾离子（K+）通道有助于调节细胞增殖和凋亡，也参与肿瘤的发生和恶性生长。我们采用免疫组织化学分析方法，研究了四种K+通道（GIRK1，即G蛋白内向整流钾通道1；Ca2+激活钾通道，K Ca 1.1；电压门控钾通道，KV 1.1和KV 1.3）以及抗凋亡蛋白Bcl2在正常乳腺组织和癌组织中的表达情况，并将其表达与临床病理数据进行比较。GIRK1在癌组织中过表达。相比之下，KV 1.1和KV 1.3在癌组织中的表达较少。Bcl-2在两种组织中的表达相似。关于临床病理数据，观察到K Ca 1.1通道与雌激素受体（ER）表达之间存在相关性。GIRK1在乳腺癌中过表达，提示其参与增殖和肿瘤发生，可能作为一个潜在的药物靶点。K Ca 1.1通道与ER之间的相关性表明该通道参与增殖。KV 1.1和KV 1.3这两种通道表达的缺失可能与其在凋亡中的作用有关。
