Glas J, Beynon V, Bachstein B, Steckenbiller J, Manolis V, Euba A, Müller-Myhsok B, Folwaczny M
Department of Preventive Dentistry and Periodontology, University of Munich, Munich, Germany.
Tissue Antigens. 2008 Jul;72(1):21-8. doi: 10.1111/j.1399-0039.2008.01056.x. Epub 2008 May 20.
Surfactant protein (SP) D belongs to the family of collectins, which are humoral molecules of the innate immune system. Collectins belong to pattern recognition receptors and are present in plasma and on mucosal surfaces and recognize several microbial components, the pathogen-associated molecular patterns (PAMPs). While SP-A is primarily expressed in the lung, expression of SP-D is more widely detected including different mucosal surfaces and in serum. Therefore, SP-D is considered a functional candidate in chronic periodontitis. The present study sought to investigate whether plasma concentration of SP-D is altered in chronic periodontitis and whether polymorphisms within the SFTPD gene (Met11Thr, Ala160Thr and Ser270Thr) are associated with chronic periodontitis. The study population comprised 105 patients with chronic periodontitis and 122 healthy, unrelated control individuals. SP-D Plasma concentrations were determined using enzyme-linked immunosorbent assay test. Genotyping of SFTPD polymorphisms was performed by polymerase chain reaction and restriction fragment length polymorphism analysis. Plasma concentrations were significantly increased in patients with chronic periodontitis compared with the controls. The median plasma concentrations were 81.6 ng/ml in the patients and 52.6 ng/ml in the controls (P = 0.00051). In contrast, the three SFTPD polymorphisms displayed no significant association with chronic periodontitis; thus, the increased plasma concentrations were independent on the genotype. The study showed significantly increased SP-D plasma concentrations in patients with chronic periodontitis compared with healthy controls. Thus, SP-D can potentially be used as a biomarker for chronic periodontitis. As no significant associations of SFTPD gene polymorphisms could be detected, other mechanisms influencing SP-D serum/plasma expression might exist.
表面活性蛋白（SP）D属于凝集素家族，凝集素是先天性免疫系统的体液分子。凝集素属于模式识别受体，存在于血浆和黏膜表面，可识别多种微生物成分，即病原体相关分子模式（PAMPs）。虽然SP - A主要在肺中表达，但SP - D的表达在更广泛的范围内被检测到，包括不同的黏膜表面和血清中。因此，SP - D被认为是慢性牙周炎的一个功能性候选指标。本研究旨在调查慢性牙周炎患者血浆中SP - D的浓度是否发生改变，以及SFTPD基因内的多态性（Met11Thr、Ala160Thr和Ser270Thr）是否与慢性牙周炎相关。研究人群包括105例慢性牙周炎患者和122名健康、无亲缘关系的对照个体。使用酶联免疫吸附测定法测定血浆中SP - D的浓度。通过聚合酶链反应和限制性片段长度多态性分析对SFTPD基因多态性进行基因分型。与对照组相比，慢性牙周炎患者的血浆浓度显著升高。患者的血浆浓度中位数为81.6 ng/ml，对照组为52.6 ng/ml（P = 0.00051）。相比之下，三个SFTPD基因多态性与慢性牙周炎无显著关联；因此，血浆浓度的升高与基因型无关。该研究表明，与健康对照组相比，慢性牙周炎患者的血浆中SP - D浓度显著升高。因此，SP - D有可能用作慢性牙周炎的生物标志物。由于未检测到SFTPD基因多态性的显著关联，可能存在影响SP - D血清/血浆表达的其他机制。
