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从水果和树皮中分离及鉴定产乙醇酵母

Isolation and characterization of ethanol-producing yeasts from fruits and tree barks.

作者信息

Rao R S, Bhadra B, Shivaji S

机构信息

Institution Centre for Cellular and Molecular Biology, Hyderabad, India.

出版信息

Lett Appl Microbiol. 2008 Jul;47(1):19-24. doi: 10.1111/j.1472-765X.2008.02380.x. Epub 2008 May 21.

DOI:10.1111/j.1472-765X.2008.02380.x
PMID:18498317
Abstract

AIMS

Isolation and identification of yeasts converting xylose to ethanol.

METHODS AND RESULTS

A total of 374 yeasts were isolated from a variety of rotten fruits and barks of trees. Out of these, 27 yeast strains were able to assimilate xylose and produce 0.12-0.38 g of ethanol per gram of xylose. Based on phylogenetic analysis of D1/D2 domain sequence of LSU (Large Subunit) rRNA gene and phenotypic characteristics the ethanol-producing strains were identified as member(s) of the genera Pichia, Candida, Kluyveromyces, Issatchenkia, Zygosacchraomyces, Clavispora, Debaryomyces, Metschnikowia, Rhodotorula and Cryptococcus.

CONCLUSION

Yeast strains producing ethanol from xylose have been isolated from a variety of rotten fruits and barks of trees and identified.

SIGNIFICANCE AND IMPACT OF THE STUDY

Environmental isolates of yeasts which could convert xylose to ethanol could form the basis for bio-fuel production and proper utilization of xylan rich agricultural and forest wastes.

摘要

目的

分离并鉴定能将木糖转化为乙醇的酵母。

方法与结果

从多种腐烂水果和树皮中分离出374株酵母。其中,27株酵母菌株能够同化木糖,每克木糖可产生0.12 - 0.38克乙醇。基于大亚基(LSU)rRNA基因D1/D2结构域序列的系统发育分析和表型特征,将产乙醇菌株鉴定为毕赤酵母属、假丝酵母属、克鲁维酵母属、伊萨酵母属、接合酵母属、克勒克酵母属、德巴利酵母属、梅奇酵母属、红酵母属和隐球菌属的成员。

结论

已从多种腐烂水果和树皮中分离并鉴定出能从木糖生产乙醇的酵母菌株。

研究的意义与影响

能够将木糖转化为乙醇的环境分离酵母菌株可为生物燃料生产以及富含木聚糖的农业和森林废弃物的合理利用奠定基础。

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