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疯狂发酵:一项生物化学实验室实验

Fermentation Gone Wild: A Biochemistry Laboratory Experiment.

作者信息

Millard Julie T, Peck Ronald F, Beachy Tina M, Hepburn Victoria L

机构信息

Departments of Chemistry and Biology, Colby College, Waterville, Maine 04901, United States.

出版信息

J Chem Educ. 2023 Jul 26;100(8):3076-3080. doi: 10.1021/acs.jchemed.3c00499. eCollection 2023 Aug 8.

DOI:10.1021/acs.jchemed.3c00499
PMID:37577454
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10413941/
Abstract

An experiment for the upper-level biochemistry laboratory is described in which students isolate a wild yeast from environmental sources and characterize the strain for its potential in the brewing industry. In addition to providing valuable experience in important biochemical techniques, this study also illustrates key principles of bioprospecting, the search for new biological sources with potential commercial or scientific value. By foraging for yeast in the wild, students explore the microbial diversity of their local environment and potentially find untapped sources of yeast that produce novel flavors and aromas. Overall, students engage with hands-on experience in bioprospecting, allowing them to appreciate the value of exploring biological diversity and its potential applications in the brewing industry.

摘要

本文描述了一个针对高年级生物化学实验室的实验,学生们从环境来源中分离出野生酵母,并对该菌株在酿造工业中的潜力进行表征。除了在重要的生化技术方面提供宝贵经验外,本研究还阐述了生物勘探的关键原则,即寻找具有潜在商业或科学价值的新生物来源。通过在野外寻找酵母,学生们探索当地环境的微生物多样性,并有可能发现尚未开发的酵母来源,这些酵母能产生新颖的风味和香气。总体而言,学生们通过亲身体验参与生物勘探,从而能够认识到探索生物多样性的价值及其在酿造工业中的潜在应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc5/10413941/5870072ee0bb/ed3c00499_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc5/10413941/5870072ee0bb/ed3c00499_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc5/10413941/5870072ee0bb/ed3c00499_0001.jpg

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本文引用的文献

1
Bootleg Biology: a Semester-Long CURE Using Wild Yeast to Brew Beer.非法生物学:一个使用野生酵母酿造啤酒的为期一学期的基于研究的本科生课程体验
J Microbiol Biol Educ. 2022 Oct 6;23(3). doi: 10.1128/jmbe.00336-21. eCollection 2022 Dec.
2
Isolation of wild yeasts from Olympic National Park and Moniliella megachiliensis ONP131 physiological characterization for beer fermentation.从奥林匹克国家公园和 Moniliella megachiliensis ONP131 中分离野生酵母,并对其进行啤酒发酵的生理特性表征。
Food Microbiol. 2022 Jun;104:103974. doi: 10.1016/j.fm.2021.103974. Epub 2021 Dec 28.
3
A Wild Yeast Laboratory Activity: From Isolation to Brewing.
一项野生酵母实验室活动:从分离到酿造
J Microbiol Biol Educ. 2021 Aug 31;22(2). doi: 10.1128/jmbe.00186-21. eCollection 2021 Fall.
4
Wild Yeast for the Future: Exploring the Use of Wild Strains for Wine and Beer Fermentation.未来的野生酵母:探索野生菌株在葡萄酒和啤酒发酵中的应用。
Front Genet. 2020 Nov 2;11:589350. doi: 10.3389/fgene.2020.589350. eCollection 2020.
5
Strains Isolated From Kombucha: Fundamental Insights, and Practical Application in Low Alcohol Beer Brewing.从康普茶中分离出的菌株:基础见解及其在低醇啤酒酿造中的实际应用。
Front Microbiol. 2020 Apr 23;11:764. doi: 10.3389/fmicb.2020.00764. eCollection 2020.
6
and its industrial applications.及其工业应用。
AIMS Microbiol. 2020 Feb 11;6(1):1-31. doi: 10.3934/microbiol.2020001. eCollection 2020.
7
Yeast bioprospecting versus synthetic biology-which is better for innovative beverage fermentation?酵母生物勘探与合成生物学——哪种方法更适合创新型饮料发酵?
Appl Microbiol Biotechnol. 2020 Mar;104(5):1939-1953. doi: 10.1007/s00253-020-10364-x. Epub 2020 Jan 18.
8
A Hands-On Guide to Brewing and Analyzing Beer in the Laboratory.实验室酿造与分析啤酒实用指南。
Curr Protoc Microbiol. 2019 Sep;54(1):e91. doi: 10.1002/cpmc.91.
9
A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms.一套全面的真菌特异性 18S rRNA 基因序列引物工具包,适用于各种研究问题和测序平台。
BMC Microbiol. 2018 Nov 20;18(1):190. doi: 10.1186/s12866-018-1331-4.
10
The beer and biofuels laboratory: A report on implementing and supporting a large, interdisciplinary, yeast-focused course-based undergraduate research experience.啤酒与生物燃料实验室:关于实施和支持一门大型、跨学科、以酵母为重点的基于课程的本科研究经历的报告。
Biochem Mol Biol Educ. 2018 May;46(3):213-222. doi: 10.1002/bmb.21111. Epub 2018 Jan 31.