Bachorik P S, Wood P D, Albers J J, Steiner P, Dempsey M, Kuba K, Warnick R, Karlsson L
Clin Chem. 1976 Nov;22(11):1828-34.
The widely used heparin/MnCl2 precipitation procedure for determination of plasma high-density lipoprotein cholesterol has been re-examined in light of recent reports that isolated preparations of the lipoprotein are only partly precipitated under the test conditions. In the present study, the procedure as applied to plasma tolerated rather wide variations in heparin and MnCl2 concentrations without significant effects on the assayed values in several plasma pools tested. The procedure was further tested on 129 individual samples by comparison with an ultracentrifugal method in which high-density lipoprotein-cholesterol is assumed to be represented by the cholesterol content of the plasma fraction of relative (to water) density greater than 1.063. Our results indicate that high-density lipoprotein is not precipitated under the test conditions when applied to unfractionated plasma.
鉴于最近有报道称，在测试条件下，分离出的脂蛋白制剂仅部分沉淀，因此对广泛用于测定血浆高密度脂蛋白胆固醇的肝素/氯化锰沉淀法进行了重新审视。在本研究中，应用于血浆的该方法在肝素和氯化锰浓度方面可耐受相当大的变化，而对所测试的几个血浆样本池中的测定值无显著影响。通过与超速离心法进行比较，对129份个体样本进一步测试了该方法。在超速离心法中，假定相对（相对于水）密度大于1.063的血浆部分中的胆固醇含量代表高密度脂蛋白胆固醇。我们的结果表明，当应用于未分级的血浆时，在测试条件下高密度脂蛋白不会沉淀。
