Warnick G R, Albers J J
Clin Chem. 1978 Jun;24(6):900-4.
We describe a modified heparin--Mn2+ procedure for high-density-lipoprotein cholesterol quantitation, especially in lipemic samples. High-density-lipoproteins may be estimated as cholesterol remaining in plasma supernates after precipitation of other lipoproteins by heparin and Mn2+ treatment. However, in lipemic samples or those from non-fasting individuals, the lower density of the precipitated chylomicrons, very-low-, and low-density-lipoproteins frequently prevents their sedimentation by the usual low-speed centrifugation, and high-density-lipoprotein cholesterol thus is overestimated in the resulting turbid supernates. Sedimentation is improved by a twofold increase in Mn2+ concentration to 92 mmol/liter. The procedure reported here produced clear supernates in more than 95% of samples tested. Any remaining turbid supernates can be cleared by a simple, convenient ultrafiltration technique. The filtration removed essentially all of the very-low- and low-density-lipoproteins without removing appreciable amounts of high-density-lipoproteins.
我们描述了一种改良的肝素-Mn2+法用于高密度脂蛋白胆固醇定量，特别是在脂血样本中。高密度脂蛋白可通过肝素和Mn2+处理沉淀其他脂蛋白后，以血浆上清液中剩余的胆固醇来估算。然而，在脂血样本或非空腹个体的样本中，沉淀的乳糜微粒、极低密度和低密度脂蛋白密度较低，常常无法通过常规低速离心沉降，因此在产生的浑浊上清液中高密度脂蛋白胆固醇被高估。将Mn2+浓度提高两倍至92 mmol/升可改善沉降。此处报道的方法在超过95%的测试样本中产生了清澈的上清液。任何剩余的浑浊上清液都可以通过一种简单便捷的超滤技术清除。过滤基本去除了所有极低密度和低密度脂蛋白，而没有去除大量的高密度脂蛋白。
