Kirstein P, Carlson K
Clin Chim Acta. 1981 Jun 18;113(2):123-34. doi: 10.1016/0009-8981(81)90146-7.
A method for the determination of the cholesterol content of human plasma high density lipoprotein (HDL) subfractions HDL2 and HDL3 is presented. The method is based on one preparative ultracentrifugal spin at density 1.125 to obtain HDL3 and heparin/manganese precipitation to obtain total HDL. From this spin in a fixed angle rotor at density 1.125 HDL3 is obtained as a bottom fraction in which cholesterol is determined. The total HDL cholesterol concentration is determined in the supernatant of the heparin/manganese precipitation of VLDL-free serum. HDL2 cholesterol is calculated as the difference between total HDL and HDL3 cholesterol. One of the advantages of this method is that falsely high HDL2 cholesterol levels due to incorporation of LP(a) containing B-protein in the HDL2 fraction is avoided. Some validation of the 1.125-spin is given. Results are presented from small samples of normal of both sexes as well as men with different kinds of hyperlipoproteinaemia.
本文介绍了一种测定人血浆高密度脂蛋白（HDL）亚组分HDL2和HDL3胆固醇含量的方法。该方法基于一次在密度1.125下的制备性超速离心以获得HDL3，以及肝素/锰沉淀以获得总HDL。在固定角度转子中于密度1.125下进行此次离心后，HDL3作为底部组分被获得，其中的胆固醇被测定。无极低密度脂蛋白（VLDL）血清的肝素/锰沉淀上清液中的总HDL胆固醇浓度被测定。HDL2胆固醇通过总HDL胆固醇与HDL3胆固醇的差值计算得出。该方法的优点之一是避免了由于HDL2组分中掺入含B蛋白的脂蛋白（a）[LP（a）]而导致的HDL2胆固醇水平假性升高。文中给出了对1.125密度离心法的一些验证。展示了来自正常男女小样本以及患有不同类型高脂蛋白血症男性的结果。
