抗利尿激素、腺苷酸环化酶、组织环磷酸腺苷与扩散性水通透性之间的相互关系。

The interrelationships between antidiuretic hormone, adenyl cyclase, tissue cyclic AMP and diffusional water permeability.

Rayson B M, Carney S, Gardner G, Morgan T

Clin Exp Pharmacol Physiol. 1976 Mar-Apr;3(2):147-57. doi: 10.1111/j.1440-1681.1976.tb00599.x.

Abstract

Physiological concentrations of antidiuretic hormone increase diffusional water permeability but not measurable cyclic AMP content in the isolated papilla of the rat's kidney. 2. Theophylline (6 mM) increases diffusional water permeability and cyclic AMP content in the isolated papilla of the rat's kidney. 3. The increase in water permeability is detected with 5 muunits.ml-1 of ADH and is maximal with 50 muunits.ml-1. The same maximum was achieved with 6 mM theophylline. 4. Cyclic AMP and dibutyryl cyclic AMP both increase water permeability, but to a lesser extent than theophylline or ADH. 5. In the presence of theophylline, ADH causes a dose related generation of tissue cyclic AMP up to a dose of 2,000,000 muunits.ml-1. 6. Adenyl cyclase is increasingly activated by ADH up to doses of 2,000,000 muunits.ml-1. 7. These results suggest that while ADH activates the adenyl cyclase system and changes water permeability there are sufficient disparities to cast doubt on an exclusive role for cyclic AMP as the second messenger.

摘要

抗利尿激素的生理浓度可增加大鼠肾脏离体乳头的扩散性水通透性，但不会使可测量的环磷酸腺苷含量增加。2. 茶碱（6 mM）可增加大鼠肾脏离体乳头的扩散性水通透性和环磷酸腺苷含量。3. 5微单位·毫升-1的抗利尿激素可检测到水通透性增加，50微单位·毫升-1时达到最大值。6 mM茶碱也能达到相同的最大值。4. 环磷酸腺苷和二丁酰环磷酸腺苷均可增加水通透性，但程度低于茶碱或抗利尿激素。5. 在茶碱存在的情况下，抗利尿激素会导致组织环磷酸腺苷产生剂量相关性增加，直至剂量达到2,000,000微单位·毫升-1。6. 腺苷酸环化酶被抗利尿激素激活，直至剂量达到2,000,000微单位·毫升-1时活性不断增加。7. 这些结果表明，虽然抗利尿激素激活腺苷酸环化酶系统并改变水通透性，但存在足够多的差异，使人怀疑环磷酸腺苷作为第二信使的唯一作用。