Carney S, Rayson B, Morgan T
Pflugers Arch. 1976 Oct 15;366(1):19-23.
The diffusional and osmotic water permeability of collecting ducts in isolated papillae of rats' kidneys were measured in papillae taken from normal and lithium pretreated rats. The diffusional water permeability of collecting ducts in papillae from normal rats in the absence of ADH was 4.1 +/- 0.2 (S.E.M.) (n = 18) muM s-1 increasing to 7.2 +/- 0.6 mum s-1 with ADH. Values obtained with lithium (10 mM) in the medium, perfusate or both and in papillae taken from lithium pretreated rats did not differ significantly from the above. The cyclic AMP content of the papillae taken from normal rats was 83 +/- 6 pm mg protein in the absence of ADH and increased to 196 +/- 12 (n = 13) with 500 mu units ml-1 ADH. Lithium 10 mM in the medium did not alter this response. Papillae from lithium pretreated rats had a similar basal level of cyclic AMP but the increment in a lithium (10 mM) medium after ADH was significantly less. These results indicate that the impaired water handling of lithium treated rats is probably not due to a failure of the membrane to increase its permeability to water after ADH. Though lithium does alter the production of cyclic AMP this is not believed to be important regarding any alteration in water permeability. We believe it is probable that lithium interferes with sodium chloride transport at some more proximal nephron segment thereby producing the syndrome of polyuria.
在取自正常大鼠和经锂预处理大鼠的肾乳头中，测量了离体大鼠肾乳头集合管的扩散性和渗透性水通透性。在无抗利尿激素（ADH）的情况下，正常大鼠肾乳头集合管的扩散性水通透性为4.1±0.2（标准误）（n = 18）μM·s⁻¹，使用ADH后增加至7.2±0.6μm·s⁻¹。在培养基、灌注液或两者中加入锂（10 mM）以及取自经锂预处理大鼠的肾乳头所获得的值与上述值无显著差异。在无ADH的情况下，取自正常大鼠的肾乳头的环磷酸腺苷（cAMP）含量为83±6 pm·mg蛋白质，加入500μ单位/ml的ADH后增加至196±12（n = 13）。培养基中加入10 mM锂不会改变这种反应。经锂预处理大鼠的肾乳头具有相似的基础cAMP水平，但加入ADH后在锂（10 mM）培养基中的增加量明显较少。这些结果表明，锂处理大鼠的水代谢受损可能不是由于膜在ADH作用后未能增加其对水的通透性。尽管锂确实会改变cAMP的产生，但这被认为与水通透性的任何改变无关。我们认为，锂可能在肾单位更近端的某个节段干扰氯化钠转运，从而产生多尿综合征。
