Yura Y, Iga H, Kondo Y, Harada K, Yanagawa T, Yoshida H, Sato M
Second Department of Oral and Maxillofacial Surgery, Tokushima University School of Dentistry, Japan.
J Oral Pathol Med. 1991 Feb;20(2):68-73. doi: 10.1111/j.1600-0714.1991.tb00892.x.
To examine the sensitivity of human oral mucosa to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infection, human gingival mucosa explants were infected with either HSV-1 or HSV-2 in vitro and the expression of virus specific antigen was examined by the immunofluorescent antibody technique. HSV-2 antigen was found in the basement membrane, basal cell layer and lower prickle cell layer. This finding was consistent with the HSV-1 infection. Electron microscopic study revealed the presence of nucleocapsids and enveloped virus particles in the basal cells of HSV-2-infected organ cultures. These findings indicate that human gingival mucosa is sensitive to infection with HSV-2, as well as HSV-1, and that the virus may replicate in the undifferentiated epithelial cells of mucosal epithelium.
为检测人类口腔黏膜对1型单纯疱疹病毒（HSV-1）和2型单纯疱疹病毒（HSV-2）感染的敏感性，体外用人牙龈黏膜外植体感染HSV-1或HSV-2，并采用免疫荧光抗体技术检测病毒特异性抗原的表达。在基底膜、基底细胞层和下部棘细胞层发现了HSV-2抗原。这一发现与HSV-1感染一致。电子显微镜研究显示，在HSV-2感染的器官培养物的基底细胞中存在核衣壳和包膜病毒颗粒。这些发现表明，人类牙龈黏膜对HSV-2感染以及HSV-1感染敏感，并且病毒可能在黏膜上皮的未分化上皮细胞中复制。
