Fulciniti Franco, Frangella Carmela, Staiano Maria, La Vecchia Franca, Botti Gerardo, Demuru Antonio, Magliulo Gabriella, Boon Mathilde E
Cytopathology Service, Department of Pathology, National Cancer Institute, Naples, Italy.
Acta Cytol. 2008 Mar-Apr;52(2):178-86. doi: 10.1159/000325476.
To exploit formalin-postfixed, air-dried smears for diagnostic immunocytochemistry (ICC).
A series of 144 cases of diagnostic fine needle cytology samples in which air-dried, supplementary smears were available was used to exploit postfixation in the process of antigenic stabilization and rescue for immunocytochemical staining.
Postfixation with formalin and with a formalin/ethanol solution gave comparable results as far as recovery and immunocytochemical detection of most monoclonal and polyclonal antibodies. The visualization of the antibody reactions was often superior to that obtained with wet-fixed slides, probably due to the interaction of slow cell dehydration with their consequent optimal flattening observed with formalin postfixation after short rehydration in physiologic saline.
Although wet fixation of cytopathologic slides in 95% ethanol represents a common standard for ICC, the usage of formalin-postfixed air-dried smears proved reliable and efficient for antigenic rescue and may enter routine usage in cytopathology laboratories. Moreover, in some instances, the visual evaluation of results was easier in the larger, well-flattened cells obtained in air-dried cells.
探索用于诊断性免疫细胞化学（ICC）的经福尔马林固定、空气干燥的涂片。
使用144例可获得空气干燥补充涂片的诊断性细针细胞学样本系列，在免疫细胞化学染色的抗原稳定和挽救过程中利用后固定。
就大多数单克隆和多克隆抗体的回收率和免疫细胞化学检测而言，用福尔马林和福尔马林/乙醇溶液进行后固定得到了可比的结果。抗体反应的可视化通常优于用湿固定玻片获得的结果，这可能是由于细胞缓慢脱水的相互作用以及随后在生理盐水中短暂复水后用福尔马林后固定观察到的最佳扁平化。
尽管在95%乙醇中对细胞病理玻片进行湿固定是ICC的常见标准，但福尔马林后固定的空气干燥涂片用于抗原挽救被证明是可靠且有效的，可能会在细胞病理学实验室进入常规使用。此外，在某些情况下，在空气干燥细胞中获得的较大、扁平化良好的细胞中对结果进行视觉评估更容易。
