Kim Beom-Jun, Kang Kyung-Min, Jung Sung Yun, Choi Hyun-Kyung, Seo Jong-Hun, Chae Ji-Hye, Cho Eun-Jung, Youn Hong-Duk, Qin Jun, Kim Seong-Tae
Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, 300 Chunchun-dong, Jangan-gu, Suwon, Kyonggi-do 440-746, Republic of Korea.
Biochem Biophys Res Commun. 2008 Jul 25;372(2):298-304. doi: 10.1016/j.bbrc.2008.05.056. Epub 2008 May 21.
Accurate chromosome segregation during cell division requires that sister chromatids are kept together by cohesin complex until anaphase, when the chromatids separate and distribute to the two daughter cells. Esco2 is an acetyltransferase that is required for the establishment of sister chromatid cohesion during S phase. Here, we report that Esco2 interacts with several component proteins of the CoREST complex, including a transcription corepressor CoREST, histone demethlyase LSD1, HDAC1, HDAC2, BRAF35, and PHF21A. Esco2 also interacts with various histone methyltransferases Suv39h1, SETDB1 and G9a. Esco2 complex purified from HeLa nuclear extract possesses histone H3 K9 methylation activity and functions as a transcription repressor. Esco2 fused to Gal4 DNA binding domain represses transcription by increasing methylation of histone H3 K9 in the promoter region. These results suggest a novel function of Esco2 in transcription repression through modulation of the chromatin structure.
细胞分裂过程中准确的染色体分离要求姐妹染色单体通过黏连蛋白复合体保持在一起，直到后期染色单体分离并分配到两个子细胞中。Esco2是一种乙酰转移酶，在S期建立姐妹染色单体黏连时是必需的。在此，我们报道Esco2与CoREST复合体的几种组成蛋白相互作用，包括转录共抑制因子CoREST、组蛋白去甲基化酶LSD1、HDAC1、HDAC2、BRAF35和PHF21A。Esco2还与各种组蛋白甲基转移酶Suv39h1、SETDB1和G9a相互作用。从HeLa细胞核提取物中纯化的Esco2复合体具有组蛋白H3 K9甲基化活性，并作为转录抑制因子发挥作用。与Gal4 DNA结合结构域融合的Esco2通过增加启动子区域组蛋白H3 K9的甲基化来抑制转录。这些结果表明Esco2通过调节染色质结构在转录抑制中具有新功能。
