Mostowfizadeh-Ghalamfarsa R, Cooke D E L, Banihashemi Z
Department of Plant Protection, College of Agriculture, Shiraz University, Bajgah, Shiraz, Iran.
Mycol Res. 2008 Jul;112(Pt 7):783-94. doi: 10.1016/j.mycres.2008.01.011. Epub 2008 Feb 12.
As part of a study to examine the phylogenetic history of the taxonomically challenging species Phytophthora cryptogea and P. drechsleri, a distinct monophyletic group of isolates, previously described as P. drechsleri or P. cryptogea, were characterised. Analysis of their rDNA ITS sequences indicated that these isolates were distinct from P. drechsleri, P. cryptogea, and all members of Phytophthora ITS clades 1-8, clustering instead alongside basal groups previously described as clades 9 and 10. This group comprised six isolates all of which were isolated from woody plants, such as pistachio (Pistacia vera, Iran and USA), fig (Ficus carica, Iran), and almond (Prunus dulcis, Greece). Analysis of sequence data from nuclear (beta-tubulin and translation elongation factor 1alpha) and mitochondrial (cytochrome c oxidase subunit I) genes confirmed the ITS-based analysis as these isolates formed a distinct monophyletic group in all NJ trees. The isolates were fast growing with a relatively high optimum growth temperature of 30 degrees C and, in most cases, rapid colony growth even at 37 degrees C. The isolates produced complex colony patterns on almost all media, especially corn meal agar (CMA). Phylogenetic analysis and examination of all the other morphological and physiological data lead us to infer that this taxon has not been described previously. As this taxon was first isolated and described from Iran we propose that this taxon be formally designated as Phytophthora parsiana.
作为一项研究的一部分，旨在探究分类学上具有挑战性的隐地疫霉（Phytophthora cryptogea）和德氏疫霉（P. drechsleri）的系统发育历史，对一个独特的单系分离菌群进行了特征描述，该菌群先前被描述为德氏疫霉或隐地疫霉。对其核糖体DNA内转录间隔区（rDNA ITS）序列的分析表明，这些分离株与德氏疫霉、隐地疫霉以及疫霉ITS进化枝1 - 8的所有成员都不同，而是与先前描述为进化枝9和10的基部类群聚集在一起。该菌群包含六个分离株，所有分离株均从木本植物中分离得到，如阿月浑子（Pistacia vera，伊朗和美国）、无花果（Ficus carica，伊朗）和杏仁（Prunus dulcis，希腊）。对来自核基因（β - 微管蛋白和翻译延伸因子1α）和线粒体基因（细胞色素c氧化酶亚基I）的序列数据进行分析，证实了基于ITS的分析结果，因为这些分离株在所有邻接法（NJ）树中都形成了一个独特的单系类群。这些分离株生长迅速，最佳生长温度相对较高，为30℃，并且在大多数情况下，即使在37℃时菌落生长也很快。这些分离株在几乎所有培养基上都产生复杂的菌落形态，尤其是在玉米粉琼脂（CMA）上。系统发育分析以及对所有其他形态和生理数据的研究使我们推断，这个分类单元以前尚未被描述过。由于这个分类单元最初是从伊朗分离并描述的，我们建议将这个分类单元正式命名为伊朗疫霉（Phytophthora parsiana）。
