Lee Seung Woo, Park Seung Bin, Choi Cheong Song
Department of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Gwahangno 335, Yuseonggu, Daejeon 305-701, Republic of Korea.
Micron. 2008 Dec;39(8):1228-34. doi: 10.1016/j.micron.2008.04.005. Epub 2008 Apr 18.
The soluble protein of hemocytes from diseased shell (HDS) of oyster, Crassostrea gigas, was shown to play a key role in the rapid growth of calcium carbonate crystals. In this study, we compared HDS extracted from regenerated (or diseased) shell with bovine carbonic anhydrase II in terms of their ability to promote the growth of calcium carbonate crystals. On the basis of scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) analysis, a high growth rate of calcium carbonate crystals was identified under artificial seawater and atmospheric temperature. The function and role of HDS extracted from regenerated shell are discussed at the molecular point as compared to aragonite-specific soluble proteins. Our findings suggest that hemocytes function as a soluble protein, with repeated GX (G: Gly, X: Asp, Asn or Glu) or negative charged amino acid domains binding calcium and specific surface features for catalyzing rapid shell regeneration.
研究表明，太平洋牡蛎患病贝壳（HDS）中的血细胞可溶性蛋白在碳酸钙晶体的快速生长中起关键作用。在本研究中，我们比较了从再生（或患病）贝壳中提取的HDS与牛碳酸酐酶II促进碳酸钙晶体生长的能力。基于扫描电子显微镜（SEM）和傅里叶变换红外光谱（FT-IR）分析，发现在人工海水和常温条件下碳酸钙晶体具有较高的生长速率。与文石特异性可溶性蛋白相比，从再生贝壳中提取的HDS的功能和作用在分子层面进行了讨论。我们的研究结果表明，血细胞作为一种可溶性蛋白发挥作用，具有重复的GX（G：甘氨酸，X：天冬氨酸、天冬酰胺或谷氨酸）或带负电荷的氨基酸结构域结合钙以及催化贝壳快速再生的特定表面特征。
